C5A ELISA 试剂盒
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- 抗原 See all C5A (C5a) ELISA试剂盒
- C5A (C5a) (Complement Component 5a (C5a))
- 抗原表位
- AA 679-755
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Complement C5a
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: N679-R755 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: N679-R755 - Top Product
- Discover our top product C5a ELISA Kit
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Complement Component 5a (C5a) ELISA Kit
C5a 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a 适用: 兔 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: Pig Colorimetric Sandwich ELISA 9.375 ng/mL - 600 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a 适用: Cow Colorimetric Sandwich ELISA 4.69 ng/mL - 300 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 anaphylatoxin-like domain.
- 板类型
- Pre-coated
- 实验流程
- mouse Complement C5a ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Complement C5a has been precoated onto 96-well plates. Standards(E.coli, N679-R755) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Complement C5a is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Complement C5a amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Complement C5a standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Complement C5a standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 37.7, Standard deviation: 1.39, CV(%): 3.7
- Sample 2: n=16, Mean(pg/ml): 118, Standard deviation: 5.43, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 32.91, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): , Standard deviation: , CV(%):
- Sample 2: n=24, Mean(pg/ml): , Standard deviation: , CV(%):
- Sample 3: n=24, Mean(pg/ml): , Standard deviation: , CV(%):
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all C5A (C5a) ELISA试剂盒
- C5A (C5a) (Complement Component 5a (C5a))
- 别名
- Complement C5a (C5a 产品)
- 背景
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Protein Function: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Background: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Synonyms: Complement C5,Hemolytic complement,Complement C5 beta chain,Complement C5 alpha chain,C5a anaphylatoxin,Complement C5 alpha' chain,C5,Hc,
Full Gene Name: Complement C5
Cellular Localisation: Secreted. - 基因ID
- 15139
- UniProt
- P06684
- 途径
- Complement System, Carbohydrate Homeostasis
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