MICB ELISA 试剂盒
Quick Overview for MICB ELISA 试剂盒 (ABIN1672867)
抗原
See all MICB ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MICB
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: A23-G298 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: A23-G298
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MHC Class I Polypeptide-Related Sequence B (MICB) ELISA Kit
MICB 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
MHC Class I Polypeptide-Related Sequence B (MICB) ELISA KitMICB 适用: 人 Colorimetric Sandwich ELISA 75 pg/mL-50 ng/mL Cell Culture Supernatant, Plasma, Serum
MHC Class I Polypeptide-Related Sequence B (MICB) ELISA KitMICB 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the MHC class I family. MIC subfamily.
Tissue Specificity: Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. .
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板类型
- Pre-coated
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实验流程
- human MICB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MICB has been precoated onto 96-well plates. Standards(NSO, A23-G298) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MICB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MICB amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MICB standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 317, Standard deviation: 20.3, CV(%): 6.4
- Sample 2: n=16, Mean(pg/ml): 1323, Standard deviation: 68.8, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 2334, Standard deviation: 100.4, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 426, Standard deviation: 32.4, CV(%): 7.6
- Sample 2: n=24, Mean(pg/ml): 1518, Standard deviation: 97.2, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 2544, Standard deviation: 150.1, CV(%): 5.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
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别名
- MICB
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背景
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Protein Function: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. .
Background: MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer(NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.
Synonyms: MHC class I polypeptide-related sequence B,MIC-B,MICB ,PERB11.2 ,
Full Gene Name: MHC class I polypeptide-related sequence B
Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum.. -
基因ID
- 4277
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UniProt
- Q29980
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途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原 See all MICB ELISA试剂盒
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