TLR3 ELISA 试剂盒
Quick Overview for TLR3 ELISA 试剂盒 (ABIN1672829)
抗原
See all TLR3 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TLR3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Expression system for standard: NSO,K27-S711
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: K27-S711
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Toll-Like Receptor 3 (TLR3) ELISA Kit
TLR3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Saliva, Tissue Homogenate
Toll-Like Receptor 3 (TLR3) ELISA KitTLR3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Toll-Like Receptor 3 (TLR3) ELISA KitTLR3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Toll-Like Receptor 3 (TLR3) ELISA KitTLR3 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the Toll-like receptor family.
Tissue Specificity: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. .
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板类型
- Pre-coated
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实验流程
- human TLR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TLR3 has been precoated onto 96-well plates. Standards (NSO,K27-S711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TLR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TLR3 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TLR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TLR3 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 824, Standard deviation: 37.08, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 2625, Standard deviation: 147, CV(%): 5.6
- Sample 3: n=16, Mean(pg/ml): 5720, Standard deviation: 366.1, CV(%): 6.4,
- Sample 1: n=24, Mean(pg/ml): 987, Standard deviation: 53.3, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 3013, Standard deviation: 213.9, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 6325, Standard deviation: 493.3, CV(%): 7.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- TLR3 (Toll-Like Receptor 3 (TLR3))
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别名
- TLR3
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背景
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Protein Function: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. .
Background: Toll-like receptor 3(TLR3) also known as CD283(cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA(dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.
Synonyms: Toll-like receptor 3,CD283,TLR3,
Full Gene Name: Toll-like receptor 3
Cellular Localisation: Endoplasmic reticulum membrane, Single-pass type I membrane protein. Endosome membrane. -
基因ID
- 7098
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UniProt
- E6Y0F1
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途径
- TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
抗原 See all TLR3 ELISA试剂盒
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