Thrombospondin 1 ELISA 试剂盒
Quick Overview for Thrombospondin 1 ELISA 试剂盒 (ABIN1672826)
抗原
See all Thrombospondin 1 (THBS1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human THBS1/TSP1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: N19-P1170 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <50pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: N19-P1170
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Thrombospondin 1 (THBS1) ELISA Kit
THBS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 人 Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 大鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 小鼠 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 1 (THBS1) ELISA KitWide Range THBS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitHigh Sensitivity THBS1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: Pig Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the thrombospondin family.
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板类型
- Pre-coated
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实验流程
- human THBS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been precoated onto 96-well plates. Standards(NSO, N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for THBS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human THBS1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 15.5, Standard deviation: 0.93, CV(%): 6
- Sample 2: n=16, Mean(ng/ml): 32, Standard deviation: 1.76, CV(%): 5.5
- Sample 3: n=16, Mean(ng/ml): 52, Standard deviation: 2.08, CV(%): 4,
- Sample 1: n=24, Mean(ng/ml): 16.3, Standard deviation: 1.27, CV(%): 7.8
- Sample 2: n=24, Mean(ng/ml): 38, Standard deviation: 2.47, CV(%): 6.5
- Sample 3: n=24, Mean(ng/ml): 64, Standard deviation: 4.61, CV(%): 7.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Thrombospondin 1 (THBS1)
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别名
- THBS1
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背景
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Protein Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). .
Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
Synonyms: Thrombospondin-1,THBS1,TSP, TSP1,
Full Gene Name: Thrombospondin-1
Cellular Localisation: Endoplasmic reticulum . Sarcoplasmic reticulum. -
基因ID
- 7057
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UniProt
- P07996
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途径
- Autophagy
抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
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