FGF7 ELISA 试剂盒
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- 抗原 See all FGF7 ELISA试剂盒
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
- 抗原表位
- AA 32-194
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF7/KGF
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: C32-T194 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <4pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: C32-T194 - Featured
- Discover our best selling FGF7 ELISA Kit
- Top Product
- Discover our top product FGF7 ELISA Kit
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Fibroblast Growth Factor 7 (FGF7) ELISA Kit
FGF7 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 7 (FGF7) ELISA KitFGF7 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 7 (FGF7) ELISA KitFGF7 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 7 (FGF7) ELISA KitFGF7 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 7 (FGF7) ELISA KitFGF7 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 7 (FGF7) ELISA KitFGF7 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 7 (FGF7) ELISA KitFGF7 适用: 猴 AA 32-194 Colorimetric Sandwich ELISA
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Epithelial cell.
- 板类型
- Pre-coated
- 实验流程
- human FGF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF7 has been precoated onto 96-well plates. Standards(E.coli, C32-T194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF7 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FGF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF7 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 6.40, CV(%): 4.1
- Sample 2: n=16, Mean(pg/ml): 645, Standard deviation: 27.09, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 1278, Standard deviation: 70.29, CV(%): 5.6,
- Sample 1: n=24, Mean(pg/ml): 147, Standard deviation: 12.35, CV(%): 8.4
- Sample 2: n=24, Mean(pg/ml): 649, Standard deviation: 39.59, CV(%): 6.1
- Sample 3: n=24, Mean(pg/ml): 1226, Standard deviation: 78.46, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all FGF7 ELISA试剂盒
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
- 别名
- FGF7 (FGF7 产品)
- 别名
- HBGF-7 ELISA Kit, KGF ELISA Kit, Kgf ELISA Kit, kgf ELISA Kit, FGF-7 ELISA Kit, fgf7 ELISA Kit, fibroblast growth factor 7 ELISA Kit, fibroblast growth factor 7 L homeolog ELISA Kit, FGF7 ELISA Kit, Fgf7 ELISA Kit, fgf7 ELISA Kit, fgf7.L ELISA Kit
- 背景
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Protein Function: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. .
Background: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Synonyms: Fibroblast growth factor 7,FGF-7,Heparin-binding growth factor 7,HBGF-7,Keratinocyte growth factor,FGF7,KGF,
Full Gene Name: Fibroblast growth factor 7
Cellular Localisation: Secreted. - 基因ID
- 2252
- UniProt
- P21781
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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