HBEGF ELISA 试剂盒
Quick Overview for HBEGF ELISA 试剂盒 (ABIN1672797)
抗原
See all HBEGF ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 15.6 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HBEGF
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: sf21
Immunogen sequence: D63-L148 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: sf21
Immunogen sequence: D63-L148
-
-
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA Kit
HBEGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
说明
-
Sequence similarities: Contains 1 EGF-like domain.
-
板类型
- Pre-coated
-
实验流程
- human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(sf21, D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HBEGF amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human HBEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HBEGF standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 92.7, Standard deviation: 5.10, CV(%): 5.5
- Sample 2: n=16, Mean(pg/ml): 369, Standard deviation: 16.97, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 23.6, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 8.26, CV(%): 8.1
- Sample 2: n=24, Mean(pg/ml): 376, Standard deviation: 27.82, CV(%): 7.4
- Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 42.02, CV(%): 6.8
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
-
别名
- HBEGF
-
背景
-
Protein Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Background: HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
Synonyms: Proheparin-binding EGF-like growth factor,Heparin-binding EGF-like growth factor,HB-EGF,HBEGF,Diphtheria toxin receptor,DT-R,HBEGF,DTR, DTS, HEGFL,
Full Gene Name: Proheparin-binding EGF-like growth factor
Cellular Localisation: Heparin-binding EGF-like growth factor: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. -
基因ID
- 1839
-
UniProt
- Q99075
-
途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原 See all HBEGF ELISA试剂盒
-
