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CD14 ELISA 试剂盒

Name: CD14 ELISA 试剂盒
SKU: ABIN1672772
Availability: OutOfStock

This 小鼠 CD14 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CD14. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN1672772

发货至: 中国

Datasheet as PDF

Quick Overview for CD14 ELISA 试剂盒 (ABIN1672772)

抗原

See all CD14 ELISA试剂盒

CD14

抗原表位

AA 18-345

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

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最低检测浓度

156 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD14

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: A18-P345

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: A18-P345
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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse CD14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD14 has been precoated onto 96-well plates. Standards(NSO, A18-P345) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD14 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD14 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(ng/ml): 0.98, Standard deviation: 0.038, CV(%): 3.9
- Sample 2: n=16, Mean(ng/ml): 3.11, Standard deviation: 0.162, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 6.23, Standard deviation: 0.355, CV(%): 5.7,
- Sample 1: n=24, Mean(ng/ml): 1.03, Standard deviation: 0.081, CV(%): 7.9
- Sample 2: n=24, Mean(ng/ml): 3.47, Standard deviation: 0.249, CV(%): 7.2
- Sample 3: n=24, Mean(ng/ml): 6.72, Standard deviation: 0.423, CV(%): 6.3
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Jiang, Chen, Xie, Ji, Liu, Qiu, Zhu, Wang: "Intrapulmonary expression of scavenger receptor and CD14 and their relation to local inflammatory responses to endotoxemia in mice." in: World journal of surgery, Vol. 27, Issue 1, pp. 1-9, (2003) (PubMed).
抗原 See all CD14 ELISA试剂盒

CD14

别名

CD14

背景

Protein Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity). .
Background: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Synonyms: Monocyte differentiation antigen CD14,Myeloid cell-specific leucine-rich glycoprotein,CD14,Cd14,
Full Gene Name: Monocyte differentiation antigen CD14
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor.

基因ID

12475

UniProt

P10810

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades