IGFBP3 ELISA 试剂盒
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Quick Overview for IGFBP3 ELISA 试剂盒 (ABIN1672736)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Mouse IGFBP-3 ELISA Kit to quantitate Mouse IGFBP3 in cell culture supernatants and serum.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G28Q-K291Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
IGFBP3 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 小鸡 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.2%, 5.4%, 6.1%
Inter-Assay Precision (CV%): 5.8%, 6.1%, 6.5% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
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别名
- IGFBP3
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背景
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Background: Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4 kDa. As a result of glycosylation, the molecular mass is 40-50KDa.
Gene Full Name: insulin-like growth factor binding protein 3
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基因ID
- 16009
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UniProt
- P47878
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途径
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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