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Interferon gamma ELISA 试剂盒

Name: Interferon gamma ELISA 试剂盒
SKU: ABIN1672731
Availability: OutOfStock

IFNG 适用: 大鼠 AA 23-156 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum

ELISA

1 image

(8 references)

产品编号 ABIN1672731

发货至: 中国

Datasheet as PDF

抗原 See all Interferon gamma (IFNG) ELISA试剂盒

Interferon gamma (IFNG)

抗原表位

AA 23-156
适用
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大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

最低检测浓度

31.2 pg/mL

应用范围

ELISA

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IFN gamma

品牌

PicoKine™

样品类型

Cell Culture Supernatant, Serum

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: E23-C156

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<6pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: E23-C156

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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: Released primarily from activated T lymphocytes.

板类型

Pre-coated

实验流程

rat IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, E23-C156) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IFN gamma amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, rat IFN gamma standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IFN gamma standard solution and each sample is measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 5.71, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 763, Standard deviation: 24.42, CV(%): 3.2
- Sample 3: n=16, Mean(pg/ml): 1479, Standard deviation: 42.89, CV(%): 2.9,
- Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 9.03, CV(%): 8.6
- Sample 2: n=24, Mean(pg/ml): 746, Standard deviation: 55.95, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 1389, Standard deviation: 79.17, CV(%): 5.7
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Chen, Wu, Chen, Xie, Fang, Hu, Chen, Fu, He: "Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-κB pathway following experimental traumatic brain injury." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 143, (2018) (PubMed).

Chen, Ye, Chen, Shi, Wu, Lin, Lin, Li, Fu, Li: "Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-κB pathway dependent of HDAC3." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 150, (2018) (PubMed).

Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Bai, Yang, Zhou, Cai, Buist-Homan, Cheng, Jiang, Shen, Li, Luo, Faber, Moshage, Shi: "The protective effect of the natural compound hesperetin against fulminant hepatitis in vivo and in vitro." in: British journal of pharmacology, Vol. 174, Issue 1, pp. 41-56, (2017) (PubMed).

Bi, Bi, Zhong, Zhang, Jin, Ma, Luo: "Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 2925-2930, (2017) (PubMed).

Hu, Wang, Zhang, Peng, Su, Chang, Liu: "Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(β-amino ester) ester nanovector induces donor-specific liver allograft tolerance." in: International journal of nanomedicine, Vol. 10, pp. 4367-82, (2016) (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).
抗原 See all Interferon gamma (IFNG) ELISA试剂盒

Interferon gamma (IFNG)

别名

IFNG (IFNG 产品)

别名

IFG ELISA Kit, IFI ELISA Kit, IFN-g ELISA Kit, Ifg ELISA Kit, IFNG2 ELISA Kit, IFN-gamma ELISA Kit, IFN-G ELISA Kit, IFNG ELISA Kit, IFNgamma ELISA Kit, TCRalpha ELISA Kit, INF-G ELISA Kit, ifng ELISA Kit, interferon gamma ELISA Kit, interferon, gamma 1-2 ELISA Kit, IFNG ELISA Kit, Ifng ELISA Kit, ifng1-2 ELISA Kit

背景

Protein Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Synonyms: Interferon gamma,IFN-gamma,Ifng,
Full Gene Name: Interferon gamma
Cellular Localisation: Secreted.

基因ID

25712

UniProt

P01581

途径

Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy