CNTF ELISA 试剂盒
(1 reference)Quick Overview for CNTF ELISA 试剂盒 (ABIN1672723)
抗原
See all CNTF ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CNTF
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: A2-M200 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: A2-M200
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Ciliary Neurotrophic Factor (CNTF) ELISA Kit
CNTF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF 适用: 大鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF 适用: 小鸡 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Nervous system.
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板类型
- Pre-coated
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实验流程
- rat CNTF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CNTF has been precoated onto 96-well plates. Standards(E.coli, A2-M200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CNTF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CNTF amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat CNTF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat CNTF standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 107, Standard deviation: 4.8, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 452, Standard deviation: 18.9, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 1287, Standard deviation: 50.2, CV(%): 3.9,
- Sample 1: n=24, Mean(pg/ml): 193, Standard deviation: 16.2, CV(%): 8.4
- Sample 2: n=24, Mean(pg/ml): 643, Standard deviation: 52.7, CV(%): 8.2
- Sample 3: n=24, Mean(pg/ml): 1057, Standard deviation: 77.2, CV(%): 7.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).
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: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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背景
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Protein Function: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Background: Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85 % homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
Synonyms: Ciliary neurotrophic factor,CNTF,Cntf,
Full Gene Name: Ciliary neurotrophic factor
Cellular Localisation: Cytoplasm. -
基因ID
- 25707
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UniProt
- P20294
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途径
- JAK/STAT Signaling
抗原 See all CNTF ELISA试剂盒
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