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CNTF ELISA 试剂盒

CNTF 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
产品编号 ABIN6719713
发货至: 中国
  • 抗原 See all CNTF ELISA试剂盒
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2 pg/mL - 2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: AI429687, Ciliary Neurotrophic Factor, HCNTF, MGC41235, OTTHUMP00000174731, OTTMUSP00000029848

    Background: Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85 % homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

    Gene Name: CNTF

    Production: Natural and recombinant rat CNTF. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: A2-M200

    Top Product
    Discover our top product CNTF ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat CNTF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. We recommend that each rat CNTF standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all CNTF ELISA试剂盒
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    别名
    CNTF (CNTF 产品)
    别名
    GPA ELISA Kit, CNTF ELISA Kit, HCNTF ELISA Kit, AI429687 ELISA Kit, ciliary neurotrophic factor ELISA Kit, CNTF ELISA Kit, Cntf ELISA Kit
    基因ID
    25707
    NCBI登录号
    NP_037298
    UniProt
    P20294
    途径
    JAK/STAT Signaling
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