Immunoglobulin isotyping
IsoT
适用: 小鼠
Colorimetric
Cell Culture Supernatant, Purified Protein
Pre-coated
Quick Overview for Immunoglobulin isotyping (ABIN1113186)
抗原
See all Ig 试剂盒
Ig
适用
小鼠
检测方法
Colorimetric
应用范围
Isotyping (IsoT)
样品类型
Cell Culture Supernatant, Purified Protein
-
-
抗原表位
- Heavy & Light Chain
-
标记
- HRP
-
原理
- The method employs the quantitative sandwich enzyme immunoassay technique.
-
品牌
- FastELISA®
-
特异性
- Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,
-
产品特性
- The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.
-
-
Ig ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ig ELISA Kit适用: 人 Chain lambda Colorimetric Competition ELISA 0.039-10 μg/mL Plasma, Serum
-
-
说明
-
One Step ELISA 30 min total time
-
实验时间
- < 1 h
-
板类型
- Pre-coated
-
实验流程
- Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
-
试剂准备
-
dilution
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
有效期
- 12 months
-
-
- Ig
-
别名
- Immunoglobulins
-
背景
- Isotyping of mouse immunoglobulins
抗原
-
You are here:
