Ig ELISA 试剂盒
The 小鼠 Ig ELISA Kit (ABIN4993802) is a Colorimetric ELISA Kit designed to quantify 小鼠 Ig.
产品编号 ABIN4993802
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Ig ELISA 试剂盒 (ABIN4993802)
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See all Ig ELISA试剂盒
Ig
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Ig Isotyping.
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Analytical Method
- Quantitative
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特异性
- IgG
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Ig ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ig ELISA Kit适用: 人 Chain lambda Colorimetric Competition ELISA 0.039-10 μg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- Ig
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背景
- Antibody isotyping is a critical and beneficial aspect of hybridoma development. This kit can identify six immunoglobulin heavy chain isotypes and two light chain isotypes in mice: IgA, IgM, IgG1, IgG2a, IgG2b, IgG3, kappa chain and lambda chain. It can accurately and specifically identify which heavy and light chain of the hybridoma samples is producing and if it is monoclonal or not. This kit is a powerful tool to isolate and characterize each potential clone.
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