Prostate Specific Antigen ELISA 试剂盒
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- 抗原 See all Prostate Specific Antigen (PSA) ELISA试剂盒
- Prostate Specific Antigen (PSA)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 灵敏度
- < 10 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human Kallikrein 3 antibody 2. Lyophilized Human Kallikrein 3 standards: 2 tubes (20ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human Kallikrein 3 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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Prostate Specific Antigen (PSA) ELISA Kit
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Prostate Specific Antigen (PSA) ELISA KitPSA 适用: 人 AA 18-261 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Kallikrein 3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Kallikrein 3 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Kallikrein 3 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Kallikrein 3 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all Prostate Specific Antigen (PSA) ELISA试剂盒
- Prostate Specific Antigen (PSA)
- 别名
- Kallikrein 3 / PSA (PSA 产品)
- 别名
- APS ELISA Kit, KLK2A1 ELISA Kit, PSA ELISA Kit, hK3 ELISA Kit, KLK3 ELISA Kit, Klk1c10l2 ELISA Kit, RSGK-50 ELISA Kit, RSKG-50 ELISA Kit, rGK-3 ELISA Kit, Kal-3 ELISA Kit, Kal3 ELISA Kit, Klk3 ELISA Kit, AAP-S ELISA Kit, MP100 ELISA Kit, Psa ELISA Kit, R74825 ELISA Kit, goku ELISA Kit, kallikrein related peptidase 3 ELISA Kit, kallikrein 3 ELISA Kit, kallikrein 1-related peptidase C3 ELISA Kit, kallikrein B, plasma 1 ELISA Kit, aminopeptidase puromycin sensitive ELISA Kit, KLK3 ELISA Kit, Klk1c3 ELISA Kit, Klkb1 ELISA Kit, Npepps ELISA Kit
- 背景
- Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a single-chain glycoprotein with a molecular mass of about 33 kD that may function normally in the liquefaction of seminal coagulum. The PSA gene spans about 6 kb and contains 5 exons. It is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is produced for the ejaculate, where it liquefies semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving cervical mucus, allowing the entry of sperm into the uterus.
- 途径
- Complement System
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