GST 抗体
(Glutathione S Transferase (GST))
The GST tag is widely used in recombinant protein expression and purification. GST antibodies used to detect GST tags (e.g. in Western Blot) serve several purposes:
- Protein Detection: Antibodies against GST tag are used to detect the presence of GST Fusion proteins in various samples, including cell lysates and purified protein preparations. This is commonly done through techniques like Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry (IHC) - with Western Blot being the predominant method.
- Protein Purification: GST is often used as an affinity tag for protein purification. An antibody against GST can be used to verify the successful purification of a GST fusion protein by detecting its presence in eluted fractions.
- Immunoprecipitation: Antibodies against GST can be used to immunoprecipitate GST Fusion proteins from complex mixtures, allowing researchers to isolate and study specific protein interactions and complexes.
- Cellular Localization: Immunofluorescence techniques (IF) can use anti-GST antibodies to determine the subcellular localization of GST-tagged proteins within cells.
- Enzyme Activity Assays: GST activity can be measured using enzyme activity assays, and anti-GST antibodies can be employed in these assays to detect the activity of GST-fused proteins.
- Protein-Protein Interaction Studies: Anti-GST antibodies can be used in co-immunoprecipitation (Co-IP) experiments to study protein-protein interactions involving GST-fused proteins.
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Latest Publications for our GST 抗体
: "Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity." in: Nature communications, Vol. 11, Issue 1, pp. 5746, (2020) (PubMed).: "NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies." in: mAbs, Vol. 12, Issue 1, pp. 1686319, (2020) (PubMed).
: "Vagus Nerve Stimulation Alleviates Hepatic Ischemia and Reperfusion Injury by Regulating Glutathione Production and Transformation." in: Oxidative medicine and cellular longevity, Vol. 2020, pp. 1079129, (2020) (PubMed).
: "Inosine induces context-dependent recoding and translational stalling." in: Nucleic acids research, Vol. 47, Issue 1, pp. 3-14, (2019) (PubMed).
: "Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening." in: Methods in molecular biology (Clifton, N.J.), Vol. 1555, pp. 437-451, (2018) (PubMed).
: "Brain Region and Isoform-Specific Phosphorylation Alters Kalirin SH2 Domain Interaction Sites and Calpain Sensitivity." in: ACS chemical neuroscience, Vol. 8, Issue 7, pp. 1554-1569, (2018) (PubMed).
: "ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis." in: Nature communications, Vol. 9, Issue 1, pp. 2702, (2018) (PubMed).
: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).
: "Protective effect of dioscin against doxorubicin-induced cardiotoxicity via adjusting microRNA-140-5p-mediated myocardial oxidative stress." in: Redox biology, Vol. 16, pp. 189-198, (2018) (PubMed).
: "Local detection of PtdIns3P at autophagosome biogenesis membrane platforms." in: Autophagy, Vol. 13, Issue 9, pp. 1602-1612, (2018) (PubMed).
Aliases for GST 抗体
glutathione S-transferase kappa 1 (GSTK1) 抗体microsomal glutathione S-transferase 1 (Mgst1) 抗体
hematopoietic prostaglandin D synthase (Hpgds) 抗体
1500002K10Rik 抗体
GST 抗体
Gst 抗体
GST13 抗体
GST 13-13 抗体
GST13-13 抗体
GSTK1-1 抗体
hGSTK1 抗体
Ptgds2 抗体
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