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Human Monoclonal GCLC Primary Antibody for IF, ELISA - ABIN561050
Hardwick, Fisher, Canet, Lake, Cherrington: Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease. in Drug metabolism and disposition: the biological fate of chemicals 2010
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Cow (Bovine) Polyclonal GCLC Primary Antibody for IHC, WB - ABIN2785780
Bardag-Gorce, Oliva, Lin, Li, French, French: Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. in Experimental and molecular pathology 2011
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Arabidopsis thaliana Polyclonal GCLC Primary Antibody for IL, WB - ABIN190704
Ghanta, Bhattacharyya, Sinha, Banerjee, Chattopadhyay: Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. in Planta 2011
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Cow (Bovine) Polyclonal GCLC Primary Antibody for IP, WB - ABIN4313738
Theodoratos, Blackburn, Cappello, Tummala, Dahlstrom, Board: Dichloroacetic acid up-regulates hepatic glutathione synthesis via the induction of glutamate-cysteine ligase. in Biochemical pharmacology 2011
Cow (Bovine) Polyclonal GCLC Primary Antibody for IHC, WB - ABIN2774078
Jönsson, Jönsson, Axmon, Littorin, Broberg: Influence of glutathione-related genes on symptoms and immunologic markers among vulcanization workers in the southern Sweden rubber industries. in International archives of occupational and environmental health 2008
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Microarray analysis revealed that glutamate (显示 GRIN2A 抗体) cysteine ligasec overexpression and thus enhanced glutathione production has a broad impact on gene expression that largely affects different processes in young and old flies.
investigation of mutations in GCL modifier subunit and the GCL catalytic subunit that modify catalytic activity and lower glutathione levels
Neuronal overexpression of GCLc in a long-lived background extended mean and maximum life spans up to 50%, without affecting the rate of oxygen consumption by the flies
The reversibility of the dephosphorylation-dependent activation was indicated by the time-dependent inactivation of the in vitro activated Drosophila GCL, by preincubation with MgATP.
that the longevity effects of GCLc are dependent on dosage and that there are specific tissues (mushroom bodies, motor neurons, and transverse muscle cells) particularly sensitive to the benefits of GCLc overexpression.
GSH biosynthesis in the nucleus is associated with migration of only the GCLc subunit from the cytoplasm into the nucleus, and this migration requires the presence of an intact nuclear localization signal
gamma-GCS has a role in chemo- and radio-resistance of human hepatocellular carcinoma cells
The findings indicate that expression of the transcription factor NRF2 and its effector GCL are both profoundly deregulated in endometriotic lesions towards increased growth and fibrogenetic processes.
Taken together, our findings provide evidence that G9a (显示 EHMT2 抗体) protects head and neck squamous cell carcinomas (HNSCC)cells against chemotherapy by increasing the synthesis of GSH, and imply G9a (显示 EHMT2 抗体) as a promising target for overcoming cisplatin resistance in HNSCC
A panel consisting of IGFBP1 (显示 IGFBPI 抗体), KIM1 (显示 HAVCR1 抗体), GCLC and GSTM1 (显示 GSTM1 抗体) genes could be used in combination for early screening of CKDu, whereas these genes in addition with FN1 (显示 FN1 抗体), IGFBP3 (显示 IGFBP3 抗体) and KLK1 (显示 KLK1 抗体) could be used to monitor progression of CKDu. The regulation of these genes has to be studied on larger populations to validate their efficiency for further clinical use.
High GCLC expression is associated with chemotherapy resistance in breast cancer.
Knockdown of CD44 (显示 CD44 抗体) reduced the protein level of xCT (显示 SLC7A11 抗体), a cystine transporter, and increased oxidative stress. However, an increase in GSH was also observed and was associated with enhanced chemoresistance in CD44 (显示 CD44 抗体)-knockdown cells. Increased GSH was mediated by the Nrf2 (显示 GABPA 抗体)/AP-1 (显示 FOSB 抗体)-induced upregulation of GCLC, a subunit of the enzyme catalyzing GSH synthesis
GCLC polymorphisms correlated with brain GSH and Glu (显示 DCTN1 抗体) levels in psychosis.
NQO1 (显示 NQO1 抗体) and GCLC were both functionally sufficient to autonomously confer a tamoxifen-resistant metabolic phenotype, characterized by i) increased mitochondrial biogenesis, ii) increased ATP production and iii) reduced glutathione levels.
(i) melatonin counteracted UVR-induced alterations in the ATP synthesis and reduced free radical formation; (ii) melatonin induced the translocation of Nrf2 (显示 GABPA 抗体) transcription factor from the cytosol into the nucleus resulting in, (iii) melatonin enhanced gene expression of phase-2 antioxidative enzymes including gamma-glutamylcysteine synthetase (gamma-GCS), heme oxygenase-1 (HO-1 (显示 HMOX1 抗体)), and NADPH (显示 NQO1 抗体): quinone dehydrogenase-1 (NQO1 (显示 NQO1 抗体)...
Glutaminolysis is activated in ES2 (显示 DGCR14 抗体) and OVCAR3, though ES2 (显示 DGCR14 抗体) exclusively synthesizes amino acids and GSH. ES2 (显示 DGCR14 抗体) cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 (显示 DGCR14 抗体) to carboplatin. HNF1beta (显示 HNF1B 抗体) regulates the expression of GCLC, but not GCLM (显示 GCLM 抗体), and consequently GSH production in ES2 (显示 DGCR14 抗体)
Retinal GCLC was significantly increased in rd10 (显示 PDE6B 抗体) mice at P21 (显示 D4S234E 抗体) as well as GSSG. Our results suggest alterations in retinal GCLC content and GSH and/or its precursors in these two RP animal models. Regulation of the enzymes related to GSH metabolism and the retinal concentration of glutamate (显示 GRIN1 抗体) may be a possible target to delay especially cone death in Retinitis Pigmentosa
Data show that the catalytic subunit of glutamate cysteine ligase (Gclc)-derived glutathione buffers reactive oxygen species (ROS (显示 ROS1 抗体)), and regulates metabolic reprogramming.
To study the biological effects of low GSH levels, we disrupted its synthesis both at birth by breeding a Gclc loxP mouse with a thy1 (显示 THY1 抗体)-cre mouse and at a later age by breeding with a CaMKII (显示 CAMK2G 抗体)-ERT2 (显示 MAPK3 抗体)-Cre (FIGSKO mouse). FIGSKO mice also develop cognitive abnormalities, i.e. learning impairment and nesting behaviors based on passive avoidance, T-Maze, and nesting behavior tests
A floxed Gclc mouse was generated and crossed with a transgenic mouse expressing Cre in the lens to generate the Lens Glutathione Synthesis Knockout mouse in which de novo GSH synthesis was completely abolished in the lens.
Clinically relevant levels of TGF-beta1 (显示 TGFB1 抗体) suppresses GCLC and GCLM (显示 GCLM 抗体) expression in mouse lung.
Data show for the first time that GCLC may serve a dual role, as a surrogate marker for cellular redox state as well as malignant potential of melanoma cells.
The impacts of four clinical missense mutations on GCLC enzymatic function in vivo and in vitro, was evaluated.
tBHQ has beneficial effects on reducing hyperglycemia-induced kidney injury, which is associated with the enhanced expression of Nrf2 (显示 NFE2L2 抗体), and its downstream antioxidant HO-1 (显示 HMOX1 抗体) and gamma-GCS (显示 UGCG 抗体) in the glomeruli of diabetic mice
In first days of life luminescence measured was in all mice with distinct strain differences indicating NF-kappaB (显示 NFKB1 抗体), superoxide dismutase (显示 SOD1 抗体), gamma-glutamylcysteine synthetase, and antioxidant responsive element activity.
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
glutamate--cysteine ligase catalytic subunit
, glutamate-cysteine ligase, catalytic subunit
, gamma-Glutamylcysteine synthetase
, gamma-Glutamylcysteine synthetase catalytic subunit
, gamma-glutamylcysteine ligase
, glutamate cysteine ligase
, glutamate-cysteine ligase
, gamma glutamylcysteine synthetase
, glutamate--cysteine ligase, chloroplastic
, GCS heavy chain
, gamma-glutamylcysteine synthetase
, gamma GCS-HS
, gamma-glutamylcysteine synthetase heavy subunit
, Glutamylcysteine gamma synthetase light chain
, glutamate-cysteine ligase catalytic subunit