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SARS-CoV-2 Spike Antibodies

The ongoing SARS-CoV-2 pandemic has created urgent needs for intervention strategies to control the crisis. The spike (S) protein of the virus forms a trimer and catalyzes fusion between viral and target cell membranes - the first key step of viral infection.⁽¹⁾ The S protein is the most likely target of neutralizing antibodies as it is the main trans-membrane glycoprotein responsible for receptor-binding and virion entry.⁽²⁾ S protein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit).⁽³⁾ The S1 subunit contains a receptor-binding domain (RBD) responsible for binding to the host cell receptor ACE2. NEW: Discover our selection of neutralizing antibodies!

References

Cai Y, Zhang J, Xiao T, Peng H, Sterling SM, Walsh RM, Rawson S, Rits-Volloch S, Chen B. Distinct conformational states of SARS-CoV-2 spike protein. bioRxiv [Preprint]. 2020 May 17:2020.05.16.099317. doi: 10.1101/2020.05.16.099317. PMID: 32511405; PMCID: PMC7263552.
Su QD, Yi Y, Zou YN, Jia ZY, Qiu F, Wang F, Yin WJ, Zhou WT, Zhang S, Yu PC, Bi SL, Shen LP, Wu GZ. The biological characteristics of SARS-CoV-2 spike protein Pro330-Leu650. Vaccine. 2020 Jul 6;38(32):5071-5075. doi: 10.1016/j.vaccine.2020.04.070. Epub 2020 Apr 30. PMID: 32513514; PMCID: PMC7190498.
Walls AC, Park YJ, Tortorici MA, Wall A, McGuire AT, Veesler D. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell. 2020 Apr 16;181(2):281-292.e6. doi: 10.1016/j.cell.2020.02.058. Epub 2020 Mar 9. PMID: 32155444; PMCID: PMC7102599.

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Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)

适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Delta, SARS CoV-2 Omicron ELISA, IF, Crys, SPR, mIHC 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546)

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546)

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)).

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)).

Biolayer interferometry was used to determine dissociation constants of SARS-BLOCK™ peptides associated with IgG neutralizing antibody (ABIN6952546).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (Trimer) protein (ABIN6952670).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (P.

Surface expression and recognition of minispike by COVID-19 patient serum.

VeroE6 cells infected over night with VSVDeltaG-minispike-eGFP (green) are recognized by IgG of S ELISA-positive patients in contrast to a control replicon expressing blue fluorescent protein (VSVDeltaG-BFP).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Mutations (indicated in orange) in the SARS-CoV-2 S Protein Receptor Binding Domain (RBD) in variants of concern (grey) and variants of note (mint).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

17 images

8 references

Independent Validation

1 validation

产品编号 ABIN6952546

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Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)

适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, Neut 宿主: Human Monoclonal unconjugated Recombinant Antibody

ELISA of the indicated samples against SARS CoV-2 S1 antigen (top) or RBD antigen (bottom).

Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (ABIN6952616) was detected by SARS-CoV-2 Inhibitor screening Kit (ABIN6952717) with a half maximal inhibitory concentration (IC50) of 1.

Immobilized SARS-CoV-2 S1 protein, His Tag (ABIN6952623) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (ABIN6952616) with a linear range of 0.

Anti-SARS-CoV-2 RBD Neutralizing Antibody (ABIN6952616), Human IgG1 on SDS-PAGE under reducing (R) condition.

Serial dilutions of Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (ABIN6952616) was detected by Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit with a half maximal inhibitory concentration (IC50) of 0.

Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody (ABIN6952616) was incubated with SARS-CoV-2 pseudotyped virus at 37 ℃ for 1 hour.

FACS analysis shows that the binding of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat.

FACS analysis shows that the binding of SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (ABIN6952625) to Vero E6 cells surface ACE2 was inhibited by increasing concentration of Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (ABIN6952616).

Binding of SAD-S35, ACE2, and CR3022 to purified S proteins measured with BioLayer Interferometry, showing the initial slope V0 at the start of binding.

ELISA of the indicated samples (indicated by the color) against the indicated antigens (different shapes).

10 images

7 references

产品编号 ABIN6952616

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SARS-CoV-2 Spike 抗体 (C-Term)

适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated

ELISA Test Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody, ABIN1030641 (1 μg/mL).

Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike in 293T Cells.

Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ABIN1030641, 0.

Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ABIN1030641, 0.

IHC/IF Validation in COVID-19 Patient Sample (Source: Nuovo et al.

IF Validation of SARS-CoV2 Spike in COVID-19 Patient Lung (Source: Magro et al.

IF Validation of SARS-CoV2 Spike in COVID-19 Patient Skin (Source: Magro et al.

IHC Validation of SARS-CoV2 Spike in the Nasopharyngeal Swab Sample of the COVID-19 Patient.

IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Lung.

IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Brain.

Overexpression Validation in Spike Transfected 293 Cells.

11 images

9 references

产品编号 ABIN1030641

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SARS-CoV-2 Spike 抗体

适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta, SARS CoV-2 Gamma, SARS CoV-2 Alpha ELISA, WB 宿主: 兔 Polyclonal unconjugated

Direct ELISA of SARS-CoV-2 variant proteins with anti-SARS-CoV-2 Spike S1 antibody ABIN6952962.

Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike (cleavage site) in 293 Transfected CellsImmunofluorescent analysis of 4 % paraformaldehyde-fixed Spike transfected 293cells labeling SARS-CoV-2 (COVID-19) Spike (cleavage site) with ABIN6952962 at 10 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Overexpression Validation in Spike Transfected 293 Cells Loading: 10 μg per lane of 293 cell lysate.

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (97-087).

ELISA Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant ProteinAntibodies: SARS-CoV-2 (COVID-19) Spike antibody (cleavage site), ABIN6952962 (1 μg/mL).

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein, 10-305.

ELISA Validation with SARS-CoV-2 (COVID-19) Spike Recombinant ProteinAntibodies: SARS-CoV-2 (COVID-19) Spike antibody (cleavage site), ABIN6952962.

ELISA Validation of SARS-CoV-2 Spike Antibody Pair A sandwich ELISA was performed using the anti-SARS-COV-2 Spike S1 antibodies ABIN6952962 (10 μg/mL ) as the capture antibody.

8 images

产品编号 ABIN6952962

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Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)

适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa ELISA, IF, Crys, SPR 宿主: 兔 Chimeric CR3022 unconjugated Recombinant Antibody

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin.

Mutations (indicated in red) in the SARS-CoV-2 S Protein receptor binding domain (RBD, mint) in variants of concern (light grey) and variants of note (light mint).

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells.

3 images

5 references

产品编号 ABIN6952547

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Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)

适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, Neut, GICA 宿主: Human Monoclonal A1 unconjugated single-domain Antibody (sdAb) Recombinant Antibody

The Binding Activity of SARS-CoV-2 Spike RBD Nanobody with SARS-CoV-2-S1-RBD.

GICA: In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (ABIN6953152) is clean, the detection limit can be as low as 25 ng/mL (1.

Activity: Binding signal of SARS-CoV-2 Spike RBD Nanobody (ABIN6953152) and SARS-CoV-2-S1-RBD (ABIN6953166) was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 is 1.

Neutralizing Assay: Binding signal of SARS-CoV-2 Spike RBD Nanobody (ABIN6953152) and SARS-CoV-2-S1-RBD (ABIN6953166) was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 is 0.

4 images

产品编号 ABIN6953152

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SARS-CoV-2 Spike S1 抗体

适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma ELISA, Neut 宿主: Human Chimeric AM122 unconjugated

Immobilized Human ACE2, His Tag (ABIN6952641) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody, Mouse IgG1 (ABIN6953206) and 0.

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (ABIN6953206) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (ABIN6953206) was more than 90% and the molecular weight of this protein is around 135-150 kDa verified by SEC-MALS.

Immobilized SARS-CoV-2 S1 protein, His Tag (ABIN6973257) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (ABIN6953206) with a linear range of 0.

Immobilized Anti-SARS-CoV-2 Spike S1 Antibody, chimeric mAb (ABIN6953206) at 2 μg/mL (100 μL/well) can bind S protein RBD.

5 images

产品编号 ABIN6953206

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Recombinant SARS-CoV-2 Spike 抗体 (AA 319-541)

适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM54 unconjugated Recombinant Antibody

Elisa plate pre-coated by 2 μg/ml (100μl/well) SARS-CoV-2 RBD protein can bind Rabbit Anti-SARS-CoV-2 RBD monoclonal antibody (clone:DM54) in a linear range of 0.

Competition flow cytometry assay demonstrating Rabbit anti-RBD monoclonal antibody (clone: DM54) blockade of SARS-CoV-2 (COVID-19) S protein RBD (1μg/ml, ABIN6961175) binding to Expi 293 cell line transfected with human ACE2.

2 images

产品编号 ABIN6964062

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Recombinant SARS-CoV-2 Spike 抗体

适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal AM015553 (553-15) unconjugated Recombinant Antibody

AbFlex SARS-CoV-2 Spike Antibody (rAb) (AM015553) tested by ELISA using SARS Spike protein ECD.

AbFlex SARS-CoV-2 Spike Antibody (rAb) (AM015553) tested by ELISA using SARS Spike protein RBD.

2 images

产品编号 ABIN6972674

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Recombinant SARS-CoV-2 Spike 抗体

适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: Human Monoclonal AM004414 (414-4) unconjugated Recombinant Antibody

SARS-CoV-2 Spike Antibody (clone AM004414) tested by ELISA.

SARS-CoV-2 Spike Antibody (AM004414) tested by Western blot.

2 images

产品编号 ABIN6972668

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