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Polyclonal Antibodies

多克隆抗体（pAbs）是由多个B细胞克隆产生的免疫球蛋白的异质性混合物，能够识别同一抗原上的多个表位。这种广泛的表位识别赋予其高灵敏度和稳健的信号生成能力，使其特别适用于检测低丰度蛋白或具有结构变异的靶标。

由于能够结合抗原的不同区域，多克隆抗体在表位部分变性或被遮蔽的情况下仍能保持良好性能，例如在Western blot或免疫组织化学（IHC）中。它们广泛应用于ELISA、WB、IHC、IF和免疫沉淀等实验中，适用于需要强且稳定信号检测的场景。

与此同时，其来源于动物的生产方式会导致批次间差异，从而影响长期重复性。此外，多表位结合可能导致其特异性低于单克隆或重组抗体。因此，多克隆抗体更适用于探索性研究、低表达靶标检测，或对灵敏度要求高于特异性的实验。

对于需要高度批次一致性或精确靶标区分的应用，单克隆或重组抗体通常更为合适。多克隆混合抗体（multiclonal antibodies）则通过结合广泛表位识别和更好的重复性提供了一种平衡方案。

在antibodies-online，我们的多克隆抗体产品组合包含用于ELISA、Western blot、免疫组织化学、免疫荧光和免疫沉淀的验证抗体。凭借广泛的靶标覆盖和应用验证数据，这些抗体可为多种研究应用提供可靠的蛋白检测工具。

重要靶标

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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588,520 results

Histone H4 抗体 (acLys12)

适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Histone H4 (acLys12) antibody (ABIN6254899)

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H4 (Lys12) Antibody.

ABIN6267063 staining Hela by IF/ICC.

Western blot analysis of extracts from mouse spleen, using Acetyl-Histone H4 (Lys12) Antibody.

ABIN6267063 at 1/100 staining human Brain tissue sections by IHC-P.

Western blot analysis of extracts from various samples, Acetyl-Histone H4 (Lys12) Antibody.

6 images

Independent Validation

1 validation

产品编号 ABIN6254899

100 μL (More quantities available)

发货至: 中国

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Independently Validated

Cortactin 抗体 (N-Term)

KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Cortactin (CTTN) (N-Term) antibody (ABIN2854674)

Multiplex Immunohistochemistry validation image for anti-Cortactin (CTTN) (N-Term) antibody (ABIN2854674)

WB Image Sample (30 ug of whole cell lysate) A: PC-12 7.

WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin, antibody at 1:250 dilution.

ICC/IF Image Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunofluorescent analysis.

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.

7 images

Independent Validation

1 validation

产品编号 ABIN2854674

100 μL

发货至: 中国

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Independently Validated

APP 抗体 (C-Term)

APP 适用: 人 WB, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody (ABIN2855011)

WB Image Sample(30 μg of whole cell lysate) A:Hep G2, 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP, antibody(10 μg/ml).

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm and nucleus by immunofluorescent analysis.

IHC-P Image APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.

WB Image Various whole cell extracts (30 μg) were separated by 7.

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm by confocal immunofluorescent analysis.

8 images

Independent Validation

1 validation

产品编号 ABIN2855011

100 μL

发货至: 中国

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Independently Validated

ARPC2 抗体

ARPC2 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody (ABIN2855249)

Multiplex Immunohistochemistry validation image for anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody (ABIN2855249)

WB Image ARPC2 antibody detects ARPC2 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2, antibody at 1:100 dilution.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2, antibody at 1:200 dilution.

WB Image ARPC2 antibody detects ARPC2 protein by Western blot analysis.

WB Image Sample(30 μg of whole cell lysate) A:293T B: HeLa S3, 12% SDS PAGE antibody diluted at 1:500

7 images

Independent Validation

1 validation

产品编号 ABIN2855249

100 μL

发货至: 中国

获取报价

Independently Validated

GLI3 抗体

GLI3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

WB Image GLI3 antibody detects GLI3 protein by Western blot analysis.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3, antibody at 1:500 dilution.

IHC-P Image GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:1000

Expression changes of Hedgehog signaling components.

Western Blot of GLI3 in the SLC mice.

Expression of genes and proteins related to Hh signaling in SLC mice.

10 images

Independent Validation

1 validation

产品编号 ABIN2855813

100 μL

发货至: 中国

获取报价

Independently Validated

Histone 3 抗体 (H3K9ac)

H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone H3 (H3) (H3K9ac) antibody (ABIN2667854)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone H3 (H3) (H3K9ac) antibody (ABIN2667854)

Western blot of Histone H3 acetyl Lys9 antibody (pAb).

ChIP of Histone H3 acetyl Lys9 pAb.

Histone H3 acetyl Lys9 antibody tested by dot blot analysis.

Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq.

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb.

7 images

3 references

Independent Validation

1 validation

产品编号 ABIN2668415

100 μg

发货至: 中国

获取报价

Independently Validated

Caspase 3 抗体 (AA 67-175)

CASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.

Anti-Caspase3 antibody, IHC(P) IHC(P): Mouse Intestine Tissue

Anti-Caspase3 antibody, IHC(P) IHC(P): Rat Intestine Tissue

Anti-Caspase3 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue

Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.

5 images

103 references

产品编号 ABIN3043802

100 μg

发货至: 中国

获取报价

ATP1A2 抗体 (AA 451-479)

ATP1A2 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19033 unconjugated

Immunofluorescence validation image for anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (AA 451-479) antibody (ABIN652011)

Anti-ATP1A2 Antibody (Center) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 μg per lane.

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) with MCF-7 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green).

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) western blot analysis in MCF-7 cell line lysates (15 μg/lane).

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) western blot analysis in mouse heart tissue lysates (15 μg/lane).

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).

7 images

2 references

Independent Validation

1 validation

产品编号 ABIN652011

400 μL (More quantities available)

发货至: 中国

获取报价

Independently Validated

beta Actin 抗体 (AA 2-16)

ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, ICC, IP 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting (WB) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunocytochemistry (ICC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse muscle section (dilution 1 : 500; red).

7 images

4 references

Independent Validation

1 validation

产品编号 ABIN1742508

100 μg

发货至: 中国

获取报价

Independently Validated

APP 抗体

Rockland 600-401-253 APP 适用: 人, 小鼠 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037)

Western Blot of Rabbit anti-Beta Amyloid antibody.

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH.

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C).

ELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjugated peptide of immunizing peptide.

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western Blot of Rabbit anti-Beta Amyloid Antibody.

Immunofluorescence (IF) image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037)

9 images

1 reference

Independent Validation

2 validations

产品编号 ABIN95037

100 μg

发货至: 中国

获取报价

Independently Validated

COL3A1 抗体

Rockland 600-401-105-0.1 COL3A1 适用: 人, Cow, Pig WB, IHC, ELISA, IP, FACS, DB, FM 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 references

Independent Validation

1 validation

产品编号 ABIN5596830

100 μg

发货至: 中国

获取报价

Independently Validated

Collagen IV 抗体

Rockland 600-401-106-0.1 COL4 适用: 人, Cow WB, ELISA, IHC, IP, DB, FM 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

Lamina lucida and lamina densa regenerate before new ECM deposition.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

12 images

8 references

Independent Validation

1 validation

产品编号 ABIN5596835

100 μg

发货至: 中国

获取报价

Independently Validated

RUNX3 抗体 (AA 31-130)

RUNX3 适用: 人 WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Runx3 Polyclonal Antibody, Unconjugated (ABIN1714069) at 1:200 followed by conjugation to the secondary antibody and DAB staining

5 images

Independent Validation

1 validation

产品编号 ABIN1714069

100 μL

发货至: 中国

获取报价

Independently Validated

MYCN 抗体 (AA 401-464)

MYCN 适用: 人, 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 401-464) antibody (ABIN760676)

Image provided by the Independent Validation Program (badge number 29800).

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human brain labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

1 reference

Independent Validation

1 validation

产品编号 ABIN760676

100 μL

发货至: 中国

获取报价

Independently Validated

TTF1 抗体 (AA 201-300)

TTF1 适用: 人, 大鼠, 小鼠 WB, FACS, ICC, IF (cc) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody (ABIN728713)

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Independently Validated Antibody, image provided by Science Direct, badge number 028752.

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min.

4 images

2 references

Independent Validation

1 validation

产品编号 ABIN728713

100 μL

发货至: 中国

获取报价

Independently Validated

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