GLUD1 抗体
Quick Overview for GLUD1 抗体 (ABIN99812)
抗原
See all GLUD1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4158-0100
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Supplier
- Rockland
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原理
- Glutamate Dehydrogenase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].
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产品特性
- Synonyms: rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1
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纯化方法
- Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glutamate Dehydrogenase [Bovine Liver]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glutamate Dehydrogenase 1 (GLUD1) antibody
GLUD1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 54-240) antibodyGLUD1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 65-273) antibodyGLUD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 绵羊, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 182-231) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 兔, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, ELISA, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 93-107) antibodyGLUD1 适用: 人, 小鼠, 马, Pig, 猴 WB, IHC, ELISA, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Glutamate Dehydrogenase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Glutamate Dehydrogenase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
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别名
- GLUD1
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背景
- Background: Glutamate Dehydrogenase, mitochondrial, converts L-glutamate into alpha-ketoglutarate. It plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, which is an important intermediate in the tricarboxylic acid cycle. It may be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. It is subject to allosteric regulation. It is activated by ADP, inhibited by GTP and ATP, and ADP can occupy the NADH binding site and activate the enzyme.
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基因ID
- 281785, 32880221
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UniProt
- P00366
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途径
- Positive Regulation of Peptide Hormone Secretion, Warburg Effect
抗原
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