Fluorescein 抗体 (Rhodamine)
Quick Overview for Fluorescein 抗体 (Rhodamine) (ABIN99348)
抗原
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 600-100-096
-
Supplier
- Rockland
-
原理
- Fluorescein Antibody Rhodamine Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein Conjugated IgG.
-
产品特性
- Synonyms: goat Anti-FITC antibody Rhodamine conjugation, TRITC conjugated goat anti-fluorescein antibody, TRITC conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Rhodamine conjugation
-
纯化方法
- Fluorescein Antibody Conjugated to Rhodamine was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
-
免疫原
-
Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
-
亚型
- IgG
-
Labeling Ratio
- 2.0
-
-
anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Anti-Fluorescein Rhodamine Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: >1:10,000
FLISA Dilution: >1:20,000
IF Microscopy Dilution: 1:500 - 1:2,500
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- Fluorescein
-
物质类
- Chemical
-
背景
- Background: Anti-Fluorescein Rhodamine Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
-
