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Fluorescein 抗体 (Alkaline Phosphatase (AP))

This anti- antibody is a 山羊多克隆 antibody detecting in WB, ELISA 和 IHC. Suitable for .

产品编号 ABIN99341

Supplier Product No.: 600-105-096

发货至: 中国

Quick Overview for Fluorescein 抗体 (Alkaline Phosphatase (AP)) (ABIN99341)

抗原

Fluorescein

宿主

山羊

克隆类型

多克隆

标记

45
5
4
4
2
2
1
1
1
1
1
1
1
1

Alkaline Phosphatase (AP)

应用范围

32
22
18
18
17
13
11
10
8
6
5
2
1
1
1
1

Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-105-096

Supplier

Rockland

原理

Fluorescein Antibody Alkaline Phosphatase Conjugated

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-Alkaline Phosphatase (calf intestine) and Fluorescein conjugated IgG.

产品特性

Synonyms: goat Anti-FITC antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-fluorescein antibody, alkaline phosphatase conjugation goat Anti-FITC antibody, alk phos conjugated goat anti-fluorescein antibody

纯化方法

Fluorescein Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

过滤

Sterile filtered

免疫原

Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other

亚型

IgG
anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated

anti-Fluorescein antibody
in vivo, NC 宿主: Human Monoclonal 4-4-20 (enhanced) unconjugated Recombinant Antibody

anti-Fluorescein antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated

anti-Fluorescein antibody
WB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Fluorescein antibody
WB, ELISA 宿主: 小鼠 Monoclonal DY495-004 unconjugated

anti-Fluorescein antibody (His tag)
in vivo, NC 宿主: Human Chimeric 4-4-20 (enhanced) His tag Fab fragment Recombinant Antibody

anti-Fluorescein antibody (His tag)
in vivo, NC 宿主: 小鼠 Chimeric 4-4-20 (enhanced) His tag Fab fragment Recombinant Antibody

anti-Fluorescein antibody
in vivo, NC 宿主: 大鼠 Chimeric 4-4-20 (enhanced) unconjugated Recombinant Antibody

anti-Fluorescein antibody
in vivo, NC 宿主: 大鼠 Chimeric 4-4-20 (enhanced) unconjugated Recombinant Antibody

应用备注

Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Fluorescein Antibody Alkaline Phosphatase Conjugated has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, in situ hybridization and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

有效期

12 months
抗原

Fluorescein

物质类

Chemical

背景

Background: Fluorescein Antibody Alkaline Phosphatase Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).

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