FTH1 抗体
(6 references)
Quick Overview for FTH1 抗体 (ABIN99122)
抗原
See all FTH1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-090-0100
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Supplier
- Rockland
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原理
- Ferritin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].
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产品特性
- Synonyms: rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH
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纯化方法
- Anti-Ferritin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Ferritin [Human Spleen]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Ferritin, Heavy Polypeptide 1 (FTH1) (AA 1-183) antibody
FTH1 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) antibodyFTH1 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (AA 1-181) antibodyFTH1 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (C-Term) antibodyVerified FTH1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (AA 1-183) antibodyFTH1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (AA 1-100) antibodyFTH1 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (AA 1-183) antibodyFTH1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal D4 unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (AA 58-180) antibodyFTH1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal FTH-2081 unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) (C-Term) antibodyFTH1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ferritin, Heavy Polypeptide 1 (FTH1) antibodyFTH1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Ferritin has been tested in ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:5,000 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." in: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).
: "Salinomycin kills cancer stem cells by sequestering iron in lysosomes." in: Nature chemistry, Vol. 9, Issue 10, pp. 1025-1033, (2019) (PubMed).
: "Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9." in: Cell reports, Vol. 20, Issue 10, pp. 2341-2356, (2018) (PubMed).
: "Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy." in: The EMBO journal, Vol. 36, Issue 1, pp. 42-60, (2017) (PubMed).
: "Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis." in: eLife, Vol. 4, (2016) (PubMed).
: "Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy." in: Nature, Vol. 509, Issue 7498, pp. 105-9, (2014) (PubMed).
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: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." in: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).
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- FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
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别名
- FTH1
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背景
- Background: Ferritin stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.
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基因ID
- 2495
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UniProt
- P02794
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途径
- Transition Metal Ion Homeostasis
抗原
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