HDAC1 抗体 (AA 385-396)
Quick Overview for HDAC1 抗体 (AA 385-396) (ABIN987284)
抗原
See all HDAC1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 385-396
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原理
- Hdac1 (aa385-396)
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序列
- PEDAIPEESG DE
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特异性
- This antibody may cross-react to HDAC2
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交叉反应
- Cow, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-PEDAIPEESGDE, from the internal region of the protein sequence according to NP_032254.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 55 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1 kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:1000.
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说明
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Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- HDAC1 (Histone Deacetylase 1 (HDAC1))
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别名
- Hdac1
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背景
- HDAC1, histone deacetylase 1, RP23-209C6.7, HD1, Hdac1-ps, MommeD5, RPD3, MGC102534, MGC118085, OTTMUSP00000010171
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基因ID
- 3065, 433759, 84576
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NCBI登录号
- NP_032254
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途径
- Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis
抗原
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