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DNASE1 抗体

This anti-DNASE1 antibody is a 兔 多克隆 antibody detecting DNASE1 in WB, ELISA 和 IP. Suitable for Cow.
Rockland
产品编号 ABIN98343
Supplier Product No.: 200-4161-0100
发货至: 中国

Quick Overview for DNASE1 抗体 (ABIN98343)

抗原

See all DNASE1 抗体
DNASE1 (Deoxyribonuclease I (DNASE1))

适用

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Cow

宿主

  • 68
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  • 1

克隆类型

  • 71
  • 15
多克隆

标记

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  • 1
  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This DNASE1 antibody is un-conjugated

应用范围

  • 73
  • 30
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Western Blotting (WB), ELISA, Immunoprecipitation (IP)
  • Supplier Product No.

    200-4161-0100

    Supplier

    Rockland

    原理

    Deoxyribonuclease I Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease A [Bovine Pancreas].

    产品特性

    Synonyms: rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

    纯化方法

    Anti-Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    免疫原

    Immunogen: Deoxyribonuclease A [Bovine Pancreas]

    Immunogen Type: Native Protein

    亚型

    IgG
  • 应用备注

    Application Note: Anti-Deoxyribonuclease I has been tested by western blot and is suitable to be assayed against 1.0 μg of Deoxyribonuclease A [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    有效期

    12 months
  • 抗原

    DNASE1 (Deoxyribonuclease I (DNASE1))

    别名

    DNASE1, DNL1

    背景

    Background: Deoxyribonuclease I seems to be involved in cell death by apoptosis, among other functions. It binds specifically to G-actin and blocks actin polymerization.

    基因ID

    282217

    NCBI登录号

    NP_776959

    UniProt

    P00639
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