PLK1 抗体 (AA 331-508)
(2 references)Quick Overview for PLK1 抗体 (AA 331-508) (ABIN969580)
抗原
See all PLK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 331-508
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原理
- PLK1 Antibody
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纯化方法
- Purified antibody
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免疫原
- Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.
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亚型
- IgG1
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anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C1 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6C6 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬, 猴 WB, IF, IP, FACS 宿主: 小鼠 Monoclonal 8C12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 201-300) antibodyPLK1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibodyPLK1 适用: 人 WB, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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ELISA: 1/10000
ICC: 1/50
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified antibody in PBS with 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." in: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, (2011) (PubMed).
: "Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein." in: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 14, pp. 2166-74, (2011) (PubMed).
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: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." in: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, (2011) (PubMed).
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- PLK1 (Polo-Like Kinase 1 (PLK1))
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别名
- PLK1
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背景
- PLK1 is critical for the initiation of centrosome maturation. Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1, -2 and -3 are ubiquitously expressed, whereas PLK4 is restricted to a few tissues including the testes and the thymus. The mRNA and protein expression of PLK1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. Furthermore, PLK3 is a much more stable protein than PLK1, -2 or -4. PLK1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 overexpression correlates with cellular proliferation and poor prognosis. PLK2 and PLK3 are involved in checkpoint-mediated cell cycle arrest to ensure genetic stability. Loss-of-function mutations in these enzymes can lead to oncogenic transformation.
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分子量
- 68 kDa
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基因ID
- 5347
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UniProt
- P53350
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途径
- Cell Division Cycle, M Phase
抗原
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