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Nanog 抗体 (AA 20-166)

This anti-Nanog antibody is a 小鼠 单克隆 antibody detecting Nanog in WB, ELISA 和 ICC. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN969308
发货至: 中国

Quick Overview for Nanog 抗体 (AA 20-166) (ABIN969308)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

克隆位点

1E6C4
  • 抗原表位

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    AA 20-166

    原理

    NANOG Antibody

    纯化方法

    Ascitic fluid

    免疫原

    Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

    亚型

    IgG1
  • 应用备注

    ELISA: 1/10000

    ICC: 1/200 - 1/1000

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Ascitic fluid containing 0.03 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Freberg, Dahl, Timoskainen, Collas: "Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract." in: Molecular biology of the cell, Vol. 18, Issue 5, pp. 1543-53, (2007) (PubMed).

  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    NANOG

    背景

    NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52 % overall amino acid identity with the mouse protein and 85 % identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

    分子量

    35 kDa

    基因ID

    79923

    UniProt

    Q9H9S0

    途径

    Stem Cell Maintenance
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