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Nanog 抗体 (AA 101-200)

Name: Nanog 抗体 (AA 101-200)
SKU: ABIN2177828
Availability: OutOfStock

NANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (ABIN2177828) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 101-200))

IHC (p)

NIH/3T3 lysates probed with Nanog Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (Nanog 抗体 (AA 101-200))

A431 lysates probed with Nanog Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (Nanog 抗体 (AA 101-200))

Formalin-fixed and paraffin embedded rat embryo labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (ABIN2177828) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 101-200))

IHC (p)

Formalin-fixed and paraffin embedded rat testis labeled with Anti-Nanog Polyclonal Antibody, Unconjugated (ABIN2177828) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 101-200))

IHC (p)

5 images

(1 reference)

产品编号 ABIN2177828

发货至: 中国

Datasheet as PDF

抗原 See all Nanog (NANOG) 抗体

Nanog (NANOG) (Nanog Homeobox (NANOG))
抗原表位
16

15

12

10

8

7

7

7

7

6

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-200
适用
132

58

22

4

4

4

3

3

2

2

1

1

1
人, 小鼠, 大鼠
宿主
122

15

3

1
兔
Compatible Secondaries
克隆类型
117

24
多克隆
标记
76

9

8

8

7

5

3

3

3

3

3

3

2

2

2

2

2
This Nanog antibody is un-conjugated
应用范围
110

59

43

43

25

25

19

18

17

16

7

7

5

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Nanog

亚型

IgG

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anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-305) antibody
NANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (N-Term) antibody
NANOG 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Nasr, Farghaly, Elsaba, El-Mokhtar, Radwan, Elsabahy, Abdelkareem, Fakhry, Mousa: "Resistance of primary breast cancer cells with enhanced pluripotency and stem cell activity to sex hormonal stimulation and suppression." in: The international journal of biochemistry & cell biology, Vol. 105, pp. 84-93, (2019) (PubMed).
抗原

Nanog (NANOG) (Nanog Homeobox (NANOG))

别名

Nanog (NANOG 产品)

别名

2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

背景

Synonyms: ENK, ecat4, 2410002E02Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog
Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

基因ID

71950

UniProt

Q80Z64

途径

Stem Cell Maintenance