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GAPDH 抗体

Name: GAPDH 抗体
Brand: antibodies-online
SKU: ABIN969164
Availability: OutOfStock

This anti-GAPDH antibody is a 小鼠单克隆 antibody detecting GAPDH in WB, ELISA, IHC 和 ICC. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 4+ publications.

Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. (GAPDH 抗体)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining. (GAPDH 抗体)

IHC

Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. (GAPDH 抗体)

3 images

(4 references)

产品编号 ABIN969164

发货至: 中国

Quick Overview for GAPDH 抗体 (ABIN969164)

抗原

See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This GAPDH antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

克隆位点

1A10

Discover our top product GAPDH Primary Antibody

原理

GAPDH Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human GAPDH expressed in E. Coli.

亚型

IgG1
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 斑马鱼 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal D1 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
ICC: 1/200 - 1/1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Xu, Xu, Gin, Nshimiyimana, Mooers, Caputi, Hannafon, Ding: "SRSF1 regulates exosome microRNA enrichment in human cancer cells." in: Cell communication and signaling : CCS, Vol. 18, Issue 1, pp. 130, (2020) (PubMed).

Hannafon, Gin, Xu, Bruns, Calloway, Ding: "Metastasis-associated protein 1 (MTA1) is transferred by exosomes and contributes to the regulation of hypoxia and estrogen signaling in breast cancer cells." in: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 13, (2019) (PubMed).

Wang, Hannafon, Wolf, Zhou, Avery, Wu, Lind, Ding: "Characterization of docosahexaenoic acid (DHA)-induced heme oxygenase-1 (HO-1) expression in human cancer cells: the importance of enhanced BTB and CNC homology 1 (Bach1) degradation." in: The Journal of nutritional biochemistry, Vol. 25, Issue 5, pp. 515-25, (2015) (PubMed).

Sumner, Huynh, Markowitz, Perhac, Hill, Coovert, Schussler, Chen, Jarecki, Burghes, Taylor, Fischbeck: "Valproic acid increases SMN levels in spinal muscular atrophy patient cells." in: Annals of neurology, Vol. 54, Issue 5, pp. 647-54, (2003) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH

背景

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer

分子量

37 kDa

基因ID

2597

UniProt

P04406