BUB1B 抗体 (AA 276-388)
(2 references)Quick Overview for BUB1B 抗体 (AA 276-388) (ABIN968853)
抗原
See all BUB1B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 276-388
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human BUBR1 aa. 276-388
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亚型
- IgG2a
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anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibody
BUB1B 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 大鼠, 猴, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 11G2 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 230-394) antibodyBUB1B 适用: 人 IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 274-525) antibodyBUB1B 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, IHC, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (C-Term) antibodyBUB1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 小鼠 WB, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8G1 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 751-850) antibodyBUB1B 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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说明
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Related Products: ABIN968535, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase." in: Oncogene, Vol. 19, Issue 40, pp. 4557-62, (2000) (PubMed).
: "Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast." in: Nature, Vol. 392, Issue 6673, pp. 303-6, (1998) (PubMed).
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: "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase." in: Oncogene, Vol. 19, Issue 40, pp. 4557-62, (2000) (PubMed).
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- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
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别名
- BUBR1
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背景
- Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately atttached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity. BUBR1 is a bub-related kinase homologous to Bub1 and MAD3. BUBR1 contains a nuclear localization signal and two highly conserved domains CD1 and CD2. CD1 is in the N-terminal region and is invoved in kinetochore localization, while CD2 is in the C-terminal region and is involved in kinase activity. BUBR1 is hyperphosphorylated during spindle checkpoint activation, and interacts with p55CDC and APC during spindle checkpoint function. In colorectal cancers, the BUBR1 gene has structural abnormalities that suggest BUBR1 is a tumor suppressor gene. Thus, BUBR1 may be a critical spindle checkpoint kinase and disruption of its function may be related to tumor formation.
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分子量
- 125 kDa
抗原
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