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LCK 抗体 (pTyr505)

Name: LCK 抗体 (pTyr505)
SKU: ABIN968844
Availability: OutOfStock

This anti-LCK antibody is a 小鼠单克隆 antibody detecting LCK in WB 和 FACS. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

Jurkat cells were either untreated (lane 1) or treated (lane 2) with Anti-CD3 for 15 minutes at 37°C. (LCK 抗体 (pTyr505))

1 image

(3 references)

产品编号 ABIN968844

发货至: 中国

Datasheet as PDF

Quick Overview for LCK 抗体 (pTyr505) (ABIN968844)

抗原

See all LCK 抗体

LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This LCK antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS)

克隆位点

4-Lck

Discover our top product LCK Primary Antibody

抗原表位
29

16

10

9

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8

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6

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3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr505

交叉反应

大鼠, 小鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Lck (pY505)

亚型

IgG1
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 3F7-F5 unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-240) antibody
LCK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 22-36) antibody
LCK 适用: 人 WB, IP, ICC, ICFC 宿主: 小鼠 Monoclonal LCK-01 unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 23-52) antibody
LCK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 468-506), (C-Term) antibody
LCK 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 480-509) antibody
LCK 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 421-509) antibody
LCK 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 39-52) antibody
Verified LCK 适用: 人, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968537, ABIN968656, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Wang, Lemay, Tsai, Veillette: "SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." in: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, (2001) (PubMed).

Hardwick, Sefton: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, (1997) (PubMed).

Lee-Fruman, Collins, Burakoff: "Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25003-10, (1996) (PubMed).
抗原

LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

别名

Lck

背景

Protein tyrosine phosphorylation is an essential step in the signal transduction cascade leading to T cell antigen receptor (TCR) activation. Lck is a protein kinase and a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface MHC class II and class I molecules, respectively. Lck is regulated by both kinases and phosphatases. Autophosphorylation at Y394 leads to conformational changes in the catalytic domain, which induces kinase activity. Repression of Lck occurs via phosphorylation at Y505, located near the carboxy-terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs. Upon phosphorylation at this site, Lck associates with the SH2 domain in the amino-terminus, thus keeping the protein biologically inactive. Lck activity and regulation is critical for activation and development of T cells.

分子量

56 kDa

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling