MAD2L2 抗体 (AA 81-180)
(2 references)Quick Overview for MAD2L2 抗体 (AA 81-180) (ABIN968795)
抗原
See all MAD2L2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 81-180
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交叉反应
- 犬
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human MAD2B aa. 81-180
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亚型
- IgG1
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anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) antibody
MAD2L2 适用: 人 WB, IF, IHC 宿主: 兔 Monoclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) (AA 1-211) antibodyMAD2L2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) antibodyMAD2L2 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Monoclonal 4D8 unconjugated Recombinant Antibody
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) (AA 198-231), (C-Term) antibodyMAD2L2 适用: 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB48484 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) (AA 1-211) antibodyMAD2L2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) (Internal Region) antibodyMAD2L2 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) (AA 1-211) antibodyMAD2L2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2) (N-Term) antibodyMAD2L2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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说明
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Related Products: ABIN968586, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, (2001) (PubMed).
: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, (2000) (PubMed).
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: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, (2001) (PubMed).
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- MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))
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别名
- MAD2B/Rev7
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背景
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Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase zeta subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression. This antibody is routinely tested by western blot analysis.
Synonyms: Mitotic Arrest Deficient-2B, Rev7 -
分子量
- 26 kDa
抗原
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