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VCP 抗体 (AA 9-130)

Name: VCP 抗体 (AA 9-130)
SKU: ABIN968778
Availability: OutOfStock

This anti-VCP antibody is a 小鼠单克隆 antibody detecting VCP in WB 和 IF. Suitable for 人, 小鼠, 大鼠, 犬和小鸡. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of VCP on a mouse cerebrum lysate. (VCP 抗体 (AA 9-130))

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2. (VCP 抗体 (AA 9-130))

2 images

(4 references)

产品编号 ABIN968778

发货至: 中国

Quick Overview for VCP 抗体 (AA 9-130) (ABIN968778)

抗原

See all VCP 抗体

VCP (Valosin Containing Protein (VCP))

适用

人, 小鼠, 大鼠, 犬, 小鸡

宿主

小鼠

克隆类型

单克隆

标记

This VCP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

18-VCP

Discover our top product VCP Primary Antibody

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AA 9-130

交叉反应

人, 大鼠, 犬, 小鸡

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse VCP aa. 9-130

亚型

IgG1
anti-Valosin Containing Protein (VCP) (N-Term) antibody
VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
KO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 125-371) antibody
VCP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 1-588) antibody
VCP 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A8 unconjugated

anti-Valosin Containing Protein (VCP) (AA 647-806) antibody
VCP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968540, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Zhang, Wang, Kajino, Greene: "VCP, a weak ATPase involved in multiple cellular events, interacts physically with BRCA1 in the nucleus of living cells." in: DNA and cell biology, Vol. 19, Issue 5, pp. 253-63, (2000) (PubMed).

Zhang, Liu, Kobayashi, Tonks: "Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17806-12, (1999) (PubMed).

Zhang, Ashendel, Becker, Morré: "Isolation and characterization of the principal ATPase associated with transitional endoplasmic reticulum of rat liver." in: The Journal of cell biology, Vol. 127, Issue 6 Pt 2, pp. 1871-83, (1995) (PubMed).

Egerton, Ashe, Chen, Druker, Burgess, Samelson: "VCP, the mammalian homolog of cdc48, is tyrosine phosphorylated in response to T cell antigen receptor activation." in: The EMBO journal, Vol. 11, Issue 10, pp. 3533-40, (1992) (PubMed).
抗原

VCP (Valosin Containing Protein (VCP))

别名

VCP

背景

ATPases that belong to the ATPase associated with different cellular activities (AAA) family are homo-oligomeric proteins that have roles in cell cycle regulation, protein degradation, organelle biogenesis, and vesicle mediated-protein transport. Valosin containing protein (VCP) is a AAA family member that is found as a hexamer in rat liver. In vitro, VCP catalyzes the hydrolysis of ATP, but this activity is distinct from classical transport ATPases. VCP protein is found primarily in the transitional elements between the rough and smooth ER (TER), but stimulation with EGF leads to VCP translocation to the nucleus. Antibodies to VCP can perturb cell-free formation of transition vesicles from isolated TER of rat liver, and can inhibit transfer of material from ER to Golgi in a reconstituted membrane system. VCP is phosphorylated after T-cell activation, and PTPH1 dephosphorylation of VCP correlates with suppression of cell growth. In addition, the N-terminal region of VCP binds to the DNA damage repair protein, BRCA1. Thus, VCP may have roles in both ER protein transport and nuclear function, which are important for cell growth and survival. This antibody is routinely tested by western blot analysis.
Synonyms: Valosin Containing Protein

分子量

97 kDa

途径

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway