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VCP 抗体 (N-Term)

Name: VCP 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2856882
Availability: OutOfStock

VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated

WB Image VCP antibody detects VCP protein by Western blot analysis. (VCP 抗体 (N-Term))

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97), antibody at 1:200 dilution. (VCP 抗体 (N-Term))

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7. (VCP 抗体 (N-Term))

IHC-P Image VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. (VCP 抗体 (N-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97), antibody at 1:500 dilution. (VCP 抗体 (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 , C: Molt-4 , D: Raji 7. (VCP 抗体 (N-Term))

PME-1 knockout reduces whole cell PP2A activity. (VCP 抗体 (N-Term))

PME-1 knockout reduces PP2A protein levels. (VCP 抗体 (N-Term))

8 images

(3 references)

产品编号 ABIN2856882

发货至: 中国

Datasheet as PDF

抗原 See all VCP 抗体

VCP (Valosin Containing Protein (VCP))
抗原表位
17

10

9

8

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
96

48

40

15

6

5

5

4

4

4

2

2

2

1

1

1

1
人
宿主
80

18

2
兔
Compatible Secondaries
克隆类型
71

29
多克隆
标记
59

8

7

4

3

3

3

3

3

3

2

2
This VCP antibody is un-conjugated
应用范围
67

39

26

20

19

14

13

12

8

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

交叉反应

犬, 人, 小鼠, 大鼠, 斑马鱼

产品特性

Rabbit Polyclonal antibody to VCP (valosin-containing protein)
VCP antibody [N1N2], N-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

亚型

IgG
anti-Valosin Containing Protein (VCP) (C-Term) antibody
KO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 125-371) antibody
VCP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 1-588) antibody
VCP 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A8 unconjugated

anti-Valosin Containing Protein (VCP) (AA 647-806) antibody
VCP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人, 小鼠 WB, ICC, FACS 宿主: 小鼠 Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP 适用: 人, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , rat brain , A549 , H1299 , HCT116

限制

仅限研究用
状态

Liquid

浓度

0.3 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Tsuji, Ohama, Nakagawa, Sato: "Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma." in: The Journal of veterinary medical science, Vol. 81, Issue 10, pp. 1424-1430, (2020) (PubMed).

Kake, Tsuji, Enjoji, Hanasaki, Hayase, Yabe, Tanaka, Nakagawa, Liu, Chang, Usui, Ohama, Sato: "The role of SET/I2PP2A in canine mammary tumors." in: Scientific reports, Vol. 7, Issue 1, pp. 4279, (2019) (PubMed).

Yabe, Tsuji, Mochida, Ikehara, Usui, Ohama, Sato: "A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation." in: FEBS open bio, Vol. 8, Issue 9, pp. 1486-1496, (2018) (PubMed).
抗原

VCP (Valosin Containing Protein (VCP))

别名

valosin containing protein (VCP 产品)

别名

ALS14 antibody, IBMPFD antibody, TERA antibody, p97 antibody, CDC48 antibody, wu:fd16d05 antibody, wu:fj63d11 antibody, p93 antibody, 3110001E05 antibody, p97/VCP antibody, 22.26 antibody, 7.12 antibody, Apple antibody, BcDNA.GM02885 antibody, BcDNA:GM02885 antibody, CG2331 antibody, DmTER94 antibody, Dmel\CG2331 antibody, I antibody, Ter94 antibody, Tera antibody, VCP antibody, VCP ATPase antibody, VCP/p97 antibody, Vcp antibody, anon-WO2004063362.65 antibody, anon-WO2004063362.67 antibody, cdc48 antibody, dTERA antibody, dVCP antibody, dmTERA antibody, dvcp antibody, l(2)03775 antibody, l(2)46CFf antibody, l(2)46CFs antibody, l(2)46Ch antibody, pTER94 antibody, ter94 antibody, vcp antibody, FANCG antibody, valosin containing protein antibody, valosin containing protein S homeolog antibody, valosin-containing protein antibody, CG2331 gene product from transcript CG2331-RA antibody, zgc:136908 antibody, transitional endoplasmic reticulum ATPase antibody, VCP antibody, vcp.S antibody, vcp antibody, Vcp antibody, Bm1_56585 antibody, LOC100136246 antibody, TER94 antibody, zgc:136908 antibody, LOC101109680 antibody

分子量

89 kDa

基因ID

7415

UniProt

P55072

途径

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway