TOPBP1 抗体 (AA 204-416)
(3 references)-
- 抗原 See all TOPBP1 抗体
- TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))
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抗原表位
- AA 204-416
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TOPBP1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human TopBP1 aa. 204-416
- 克隆位点
- 33-TopBP1
- 亚型
- IgG1
- Top Product
- Discover our top product TOPBP1 Primary Antibody
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anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1253-1522) antibody
TOPBP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) antibodyTOPBP1 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1327-1435) antibodyTOPBP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6D12 unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1-100) antibodyTOPBP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1-250) antibodyTOPBP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1-246) antibodyTOPBP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1253-1522) antibodyTOPBP1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) antibodyTOPBP1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1327-1435) antibodyTOPBP1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (Internal Region) antibodyTOPBP1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN968537, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 678-84, (2001) (PubMed).
: "Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA." in: Oncogene, Vol. 18, Issue 37, pp. 5194-203, (1999) (PubMed).
: "A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator." in: European journal of biochemistry / FEBS, Vol. 250, Issue 3, pp. 794-9, (1998) (PubMed).
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: "The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 678-84, (2001) (PubMed).
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- 抗原
- TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))
- 别名
- TopBP1 (TOPBP1 产品)
- 别名
- RGD1562949 antibody, TOPBP1 antibody, cut5 antibody, F2P24.3 antibody, F2P24_3 antibody, meiosis defective 1 antibody, TOP2BP1 antibody, 1110031N14Rik antibody, 2810429C13Rik antibody, AI256758 antibody, D430026L04Rik antibody, mKIAA0259 antibody, TopBP1-A antibody, TopBP1-B antibody, Xmus101 antibody, XtopBP antibody, mus101 antibody, top2bp1 antibody, DNA topoisomerase II binding protein 1 antibody, topoisomerase (DNA) II binding protein 1 antibody, dna topoisomerase ii binding protein 1 (IC) antibody, transcription coactivator antibody, DNA topoisomerase II binding protein 1 L homeolog antibody, Topbp1 antibody, topbp1 antibody, TOPBP1 antibody, MEI1 antibody, topbp1.L antibody
- 背景
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Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIalpha and topo IIbeta. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. DNA Topoisomerase II Binding Protein 1 (TopBP1) binds the C-terminal region of Topo IIbeta via its N-terminal region. In addition, TopBP1 contains a putative ADP-ribosylation site, two N-terminal NLS domains, and 8 repeating BRCA1 C-terminal (BRCT) domains found in DNA repair proteins, such as BRCA1, XRCC1, and Rad4. The BRCT domains of TopBP1 have been shown to bind DNA breaks, DNA nicks, and DNA termini, but not circular intact DNA. Thus, TopBP1 may localize topoisomerases to sites of DNA damage.
Synonyms: Topoisomerase II Binding Protein 1 - 分子量
- 161 kDa
- 途径
- Chromatin Binding
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