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ILK 抗体 (AA 326-452)

Name: ILK 抗体 (AA 326-452)
SKU: ABIN968662
Availability: OutOfStock

This anti-ILK antibody is a 小鼠单克隆 antibody detecting ILK in WB 和 IF. Suitable for 人, 小鼠, 大鼠, 犬和小鸡. This Primary Antibody has been cited in 5+ publications.

Western blot analysis of ILK on Hs68 cell lysate. (ILK 抗体 (AA 326-452))

1 image

(5 references)

产品编号 ABIN968662

发货至: 中国

Quick Overview for ILK 抗体 (AA 326-452) (ABIN968662)

抗原

See all ILK 抗体

ILK (Integrin-Linked Kinase (ILK))

适用

人, 小鼠, 大鼠, 犬, 小鸡

宿主

小鼠

克隆类型

单克隆

标记

This ILK antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

3-ILK

Discover our top product ILK Primary Antibody

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AA 326-452

交叉反应

小鸡, 犬, 小鼠, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human ILK aa. 326-452

亚型

IgG1
anti-Integrin-Linked Kinase (ILK) antibody
KO Validated ILK 适用: 人 WB, IHC, IHC (p), IP, IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 97-244) antibody
ILK 适用: 人, 小鼠, 猴 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3A9 unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 341-452) antibody
ILK 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 4F10 unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 301-400) antibody
ILK 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 1-260) antibody
ILK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 225-253) antibody
ILK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 11-41) antibody
ILK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (pThr173) antibody
ILK 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 1-228) antibody
ILK 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (C-Term) antibody
ILK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Nikolopoulos, Turner: "Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1568-75, (2002) (PubMed).

Nikolopoulos, Turner: "Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23499-505, (2001) (PubMed).

Persad, Attwell, Gray, Delcommenne, Troussard, Sanghera, Dedhar: "Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3207-12, (2000) (PubMed).

Delcommenne, Tan, Gray, Rue, Woodgett, Dedhar: "Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 19, pp. 11211-6, (1998) (PubMed).

Hannigan, Leung-Hagesteijn, Fitz-Gibbon, Coppolino, Radeva, Filmus, Bell, Dedhar: "Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase." in: Nature, Vol. 379, Issue 6560, pp. 91-6, (1996) (PubMed).
抗原

ILK (Integrin-Linked Kinase (ILK))

别名

ILK

背景

Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of alpha and beta subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. In addition, the interaction of ILK with the cytoplasmic domains of integrin beta1 and beta3 subunits and localization of ILK to focal adhesion plaques suggests a role for ILK in the regulation of cell adhesion. Interestingly, overexpression of ILK in epithelial cells inhibits cell adhesion and stimulates cell cycle progression and tumorigenicity. Thus, ILK regulates signal transduction in many different cell signaling pathways. This antibody is routinely tested by western blot analysis.

分子量

50 kDa

途径

Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration