Dihydrofolate Reductase 抗体 (AA 1-186)
(5 references)Quick Overview for Dihydrofolate Reductase 抗体 (AA 1-186) (ABIN968119)
抗原
See all Dihydrofolate Reductase (DHFR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-186
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交叉反应
- 小鼠, 人, 犬, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Cow DHFR aa. 1-186
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亚型
- IgG1
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anti-Dihydrofolate Reductase (DHFR) (AA 88-187) antibody
DHFR 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6G7 unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 2-187) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Monoclonal 9B2 unconjugated Recombinant Antibody
anti-Dihydrofolate Reductase (DHFR) (AA 135-164) antibodyDHFR 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-50) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 16-30), (N-Term) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49346 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-187) antibodyDHFR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, IHC (p), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20°C.
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: "Distinct pattern of E2F1 expression in human lung tumours: E2F1 is upregulated in small cell lung carcinoma." in: Oncogene, Vol. 20, Issue 14, pp. 1678-87, (2001) (PubMed).
: "HNPCC-like cancer predisposition in mice through simultaneous loss of Msh3 and Msh6 mismatch-repair protein functions." in: Nature genetics, Vol. 23, Issue 3, pp. 359-62, (1999) (PubMed).
: "Repair of benzo(a)pyrene diol epoxide- and UV-induced DNA damage in dihydrofolate reductase and adenine phosphoribosyltransferase genes of CHO cells." in: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12749-54, (1994) (PubMed).
: "Dexamethasone negatively regulates the activity of a chimeric dihydrofolate reductase/glucocorticoid receptor protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 4290-4, (1993) (PubMed).
: "The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase." in: Gene, Vol. 21, Issue 1-2, pp. 59-63, (1983) (PubMed).
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: "Distinct pattern of E2F1 expression in human lung tumours: E2F1 is upregulated in small cell lung carcinoma." in: Oncogene, Vol. 20, Issue 14, pp. 1678-87, (2001) (PubMed).
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- Dihydrofolate Reductase (DHFR)
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别名
- DHFR
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背景
- Dihydrofolate reductase (DHFR) regenerates tetrahydrofolate from dihydrofolate in the presence of NADPH. Tetrahydrofolate is the coenzyme for thymidilate synthetase in the biosynthesis of thymidine and is also critical for the synthesis of amino acids and purines. DHFR is a protein of 186 amino acids that is highly conserved among different organisms. DHFR levels change during the cell cycle, with the highest content during the G1/S transition. Expression of DHFR tightly correlates with the turnover rate of its mRNA. Because dividing cells require a continuous supply of thymidine, DHFR has been a target for anticancer drugs. The folic acid antagonists aminopterin and amethopterin (methotrexate), widely used in cancer treatments, inhibit DHFR. However, over long treatments, treated cells often amplify the DHFR gene, producing drug-resistant cells.
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分子量
- 21 kDa
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途径
- Mitotic G1-G1/S Phases
抗原
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