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Dihydrofolate Reductase 抗体 (AA 1-186)

Name: Dihydrofolate Reductase 抗体 (AA 1-186)
SKU: ABIN968119
Availability: OutOfStock

Cited in 5+ publications. This 小鼠单克隆 anti-Dihydrofolate Reductase antibody (Clone 49-DHFR) specifically detects Dihydrofolate Reductase in WB, IHC, IF 和 IP. The antibody is reactive with 人, 小鼠, 大鼠和犬 samples.

Western blot analysis of DHFR on RSV-3T3 lysate. (Dihydrofolate Reductase 抗体 (AA 1-186))

Image no. 2 for anti-Dihydrofolate Reductase (DHFR) (AA 1-186) antibody (ABIN968119) (Dihydrofolate Reductase 抗体 (AA 1-186))

Western Blotting (WB) image for anti-Dihydrofolate Reductase (DHFR) (AA 1-186) antibody (ABIN968119) (Dihydrofolate Reductase 抗体 (AA 1-186))

3 images

(5 references)

产品编号 ABIN968119

发货至: 中国

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Quick Overview for Dihydrofolate Reductase 抗体 (AA 1-186) (ABIN968119)

抗原

See all Dihydrofolate Reductase (DHFR) 抗体

Dihydrofolate Reductase (DHFR)

适用

人, 小鼠, 大鼠, 犬

宿主

小鼠

克隆类型

单克隆

标记

This Dihydrofolate Reductase antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

49-DHFR

Discover our top product DHFR Primary Antibody

抗原表位
7

6

3

3

2

2

1

1
AA 1-186

交叉反应

小鼠, 人, 犬, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Cow DHFR aa. 1-186

亚型

IgG1
anti-Dihydrofolate Reductase (DHFR) (AA 88-187) antibody
DHFR 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B10 unconjugated

anti-Dihydrofolate Reductase (DHFR) antibody
DHFR 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6G7 unconjugated

anti-Dihydrofolate Reductase (DHFR) (AA 2-187) antibody
DHFR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Dihydrofolate Reductase (DHFR) antibody
DHFR 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 9B2 unconjugated Recombinant Antibody

anti-Dihydrofolate Reductase (DHFR) (AA 1-50) antibody
DHFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Dihydrofolate Reductase (DHFR) (AA 135-164) antibody
DHFR 适用: 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Dihydrofolate Reductase (DHFR) (AA 1-187) antibody
DHFR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Dihydrofolate Reductase (DHFR) (AA 16-30), (N-Term) antibody
DHFR 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49346 unconjugated

anti-Dihydrofolate Reductase (DHFR) antibody
DHFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Dihydrofolate Reductase (DHFR) antibody
DHFR 适用: 人 WB, IHC (p), FACS, IF (p) 宿主: 兔 Polyclonal unconjugated

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Eymin, Gazzeri, Brambilla, Brambilla: "Distinct pattern of E2F1 expression in human lung tumours: E2F1 is upregulated in small cell lung carcinoma." in: Oncogene, Vol. 20, Issue 14, pp. 1678-87, (2001) (PubMed).

de Wind, Dekker, Claij, Jansen, van Klink, Radman, Riggins, van der Valk, vant Wout, te Riele: "HNPCC-like cancer predisposition in mice through simultaneous loss of Msh3 and Msh6 mismatch-repair protein functions." in: Nature genetics, Vol. 23, Issue 3, pp. 359-62, (1999) (PubMed).

Tang, Pao, Zhang: "Repair of benzo(a)pyrene diol epoxide- and UV-induced DNA damage in dihydrofolate reductase and adenine phosphoribosyltransferase genes of CHO cells." in: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12749-54, (1994) (PubMed).

Israel, Kaufman: "Dexamethasone negatively regulates the activity of a chimeric dihydrofolate reductase/glucocorticoid receptor protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 4290-4, (1993) (PubMed).

Masters, Attardi: "The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase." in: Gene, Vol. 21, Issue 1-2, pp. 59-63, (1983) (PubMed).
抗原

Dihydrofolate Reductase (DHFR)

别名

DHFR

背景

Dihydrofolate reductase (DHFR) regenerates tetrahydrofolate from dihydrofolate in the presence of NADPH. Tetrahydrofolate is the coenzyme for thymidilate synthetase in the biosynthesis of thymidine and is also critical for the synthesis of amino acids and purines. DHFR is a protein of 186 amino acids that is highly conserved among different organisms. DHFR levels change during the cell cycle, with the highest content during the G1/S transition. Expression of DHFR tightly correlates with the turnover rate of its mRNA. Because dividing cells require a continuous supply of thymidine, DHFR has been a target for anticancer drugs. The folic acid antagonists aminopterin and amethopterin (methotrexate), widely used in cancer treatments, inhibit DHFR. However, over long treatments, treated cells often amplify the DHFR gene, producing drug-resistant cells.

分子量

21 kDa

途径

Mitotic G1-G1/S Phases