PKC iota 抗体 (AA 404-587)
(5 references)Quick Overview for PKC iota 抗体 (AA 404-587) (ABIN967794)
抗原
See all PKC iota (PRKCI) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 404-587
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交叉反应
- 大鼠, 小鸡, 犬, 小鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human PKCι aa. 404-587
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亚型
- IgG2b
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anti-Protein Kinase C, iota (PRKCI) (AA 375-596) antibody
PRKCI 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, iota (PRKCI) (AA 1-587) antibodyPRKCI 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 1G11 unconjugated
anti-Protein Kinase C, iota (PRKCI) (AA 21-214) antibodyPRKCI 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, iota (PRKCI) (AA 1-100) antibodyPRKCI 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-Protein Kinase C, iota (PRKCI) (AA 176-207), (N-Term) antibodyPRKCI 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01026 unconjugated
anti-Protein Kinase C, iota (PRKCI) (Internal Region) antibodyPRKCI 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, iota (PRKCI) (AA 215-264) antibodyPRKCI 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, iota (PRKCI) (Middle Region) antibodyPRKCI 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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说明
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Related Products: ABIN968545, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "Serotonin-induced MMP-13 production is mediated via phospholipase C, protein kinase C, and ERK1/2 in rat uterine smooth muscle cells." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42830-40, (2002) (PubMed).
: "NF-kappaB/RelA transactivation is required for atypical protein kinase C iota-mediated cell survival." in: Oncogene, Vol. 20, Issue 35, pp. 4777-92, (2001) (PubMed).
: "Specific cellular proteins associate with angiotensin-converting enzyme and regulate its intracellular transport and cleavage-secretion." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23253-8, (2000) (PubMed).
: "A requirement for protein kinase C inhibition for calcium-triggered apoptosis in acute lymphoblastic leukemia cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 5, Issue 2, pp. 355-60, (1999) (PubMed).
: "Molecular cloning and characterization of PKC iota, an atypical isoform of protein kinase C derived from insulin-secreting cells." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24296-302, (1993) (PubMed).
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: "Serotonin-induced MMP-13 production is mediated via phospholipase C, protein kinase C, and ERK1/2 in rat uterine smooth muscle cells." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42830-40, (2002) (PubMed).
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- PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
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别名
- PKC iota
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背景
- The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions(V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca 2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCiota was isolated from a human kidney cDNA library. It is highly expressed in brain and lung, but it is also seen in many other tissues at lower levels. PKCiota shows the most similarity to PKCzeta , 72% overall identity. These two enzymes share a highly conserved pseudosubstrate sequence, the absence of a Ca2+-binding region, and the presence of only one zinc finger-like domain. It is thought that PKCiota has a role in the secretory response to nutrients. This antibody is routinely tested by western blot analysis.
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分子量
- 74 kDa
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途径
- Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation
抗原
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