Phosphotyrosine 抗体 (HRP)
(5 references)Our Local Distributor
北京 101111
Quick Overview for Phosphotyrosine 抗体 (HRP) (ABIN967694)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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亚型
- IgG2b
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anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 390
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应用备注
- The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.
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说明
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Related Products: ABIN968534
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol.
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储存条件
- -20 °C
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储存方法
- The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed by gel filtration chromatography. Store undiluted at -20° C.
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: "BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl." in: Oncogene, Vol. 21, Issue 20, pp. 3225-31, (2002) (PubMed).
: "Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28578-85, (2001) (PubMed).
: "Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain." in: The Journal of biological chemistry, Vol. 275, Issue 51, pp. 39867-73, (2001) (PubMed).
: "MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36043-50, (2001) (PubMed).
: "p110beta and p110delta phosphatidylinositol 3-kinases up-regulate Fc(epsilon)RI-activated Ca2+ influx by enhancing inositol 1,4,5-trisphosphate production." in: The Journal of biological chemistry, Vol. 276, Issue 20, pp. 17213-20, (2001) (PubMed).
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- Phosphotyrosine
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物质类
- Amino Acid
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背景
- Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.
抗原
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