N-Cadherin 抗体 (Extracellular Domain)
(3 references)-
- 抗原 See all N-Cadherin (CDH2) 抗体
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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抗原表位
- Extracellular Domain
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This N-Cadherin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to us for technical protocols.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human extracellular N-Cadherin domain Recombinant Protein
- 克隆位点
- 8C11
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CDH2 Primary Antibody
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anti-Cadherin 2 (CDH2) (AA 701-800) antibody
CDH2 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 犬, 猴 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2G7 unconjugated
anti-Cadherin 2 (CDH2) (AA 750-850) antibodyCDH2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 5D5 unconjugated
anti-Cadherin 2 (CDH2) antibodyKO Validated CDH2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 45-238) antibodyCDH2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 690-770) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 450-550) antibodyCDH2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Because the extracellular domain of N-Cadherin is trypsin-sensitive, it is important to avoid using trypsin to dissociate the cells to be studied.
- 说明
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Related Products: ABIN968535, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "N-cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells." in: Stem cell research, Vol. 4, Issue 2, pp. 129-39, (2010) (PubMed).
: "N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation." in: Journal of cell science, Vol. 114, Issue Pt 8, pp. 1567-77, (2001) (PubMed).
: "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin." in: The Journal of cell biology, Vol. 130, Issue 1, pp. 67-77, (1995) (PubMed).
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: "N-cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells." in: Stem cell research, Vol. 4, Issue 2, pp. 129-39, (2010) (PubMed).
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- 抗原
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
- 别名
- CD325 (CDH2 产品)
- 别名
- CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody
- 背景
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The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325). Cadherins are a family of Ca2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (alpha, beta, gamma). Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of beta-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates beta-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.
Synonyms: Cadherin-2, N-Cadherin - 分子量
- 130 kDa
- 基因ID
- 1000
- 途径
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
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