CDK4 抗体
(2 references)Quick Overview for CDK4 抗体 (ABIN967647)
抗原
See all CDK4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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交叉反应
- 小鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Recombinant Human Cdk4
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亚型
- IgG1 kappa
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anti-Cyclin-Dependent Kinase 4 (CDK4) antibody
KO Validated CDK4 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 6-295) antibodyCDK4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (N-Term) antibodyCDK4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-303) antibodyCDK4 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 4F11 unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 273-305), (C-Term) antibodyCDK4 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB03154 unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 201-303) antibodyCDK4 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 273-305) antibodyCDK4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 241-303) antibodyCDK4 适用: 人, 小鼠, 大鼠, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303), (C-Term) antibodyCDK4 适用: 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303) antibodyCDK4 适用: 人, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Applications include western blot analysis (0.5-2.0 µg/ml) and immunoprecipitation (1.0 µg/one million cells). The antibody has also been used for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections, but this application is not routinely tested. HeLa (ATCC CCL 2), 293 (ATCC CRL 1573), or NIH/3T3 (ATCC CRL 1658) cells are suggested as positive controls.
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说明
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Related Products: ABIN967389, ABIN968535
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, (1999) (PubMed).
: "Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins." in: Cell, Vol. 71, Issue 2, pp. 323-34, (1992) (PubMed).
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: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, (1999) (PubMed).
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- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
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别名
- Cdk4
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背景
- Cyclins, cyclin-dependent kinases (Cdks), and cyclin-dependent kinase inhibitors (CdkIs) are essential for cell-cycle control in eukarytotes. Cyclins, regulatory subunits, bind to cyclin-dependent kinases (Cdks), catalytic subunits, to form active cyclin-Cdk complexes. Cdk subunits by themselves are inactive and binding to a cyclin is required for their activity. Cyclins A, B1, D and E undergo periodic synthesis and degradation, thereby providing a mechanism to regulate Cdk activity throughout the cell cycle. Additionally, Cdk activity is further regulated by activating and inhibitory phosphorylations, and small proteins (p15, p16, p18, p19, p21 and p27), called inhibitors of Cdk activity, that bind to cyclins, Cdks, or cyclin- Cdk complexes. Cdk4 was originally called PSK-J3, and following demonstration of its association with D-type cyclins, became known as Cdk4. D-type cyclins also associate with Cdks 2 and 5, although Cdk4 appears to be the most abundant partner. The D-type cyclins (D1, D2, and D3) are expressed in response to growth factors or mitogens, and rapidly degrade when mitogens are withdrawn. D cyclins appear to promote G0 to G1 transitions and the rate of G1 progression. For example, cyclin D-Cdk4 and cyclin D-Cdk6 complexes phosphorylate the retinoblastoma protein (Rb) which removes the G1 phase block caused by underphosphorylated Rb. Cdk4 has a molecular weight of ~32 kD. Clone DCS-156 recognizes human and mouse Cdk4. A recombinant protein fragment from the C-terminal end of human Cdk4 was used as immunogen.
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分子量
- 32 kDa
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
抗原
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