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MAP2 抗体

Name: MAP2 抗体
SKU: ABIN967494
Availability: OutOfStock

This anti-MAP2 antibody is a 小鼠单克隆 antibody detecting MAP2 in WB, IP 和 IHC (fro). Suitable for 人, 大鼠, Cow, Quail 和 Frog. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of MAP2. (MAP2 抗体)

Image no. 2 for anti-Microtubule-Associated Protein 2 (MAP2) antibody (ABIN967494) (MAP2 抗体)

Western Blotting (WB) image for anti-Microtubule-Associated Protein 2 (MAP2) antibody (ABIN967494) (MAP2 抗体)

3 images

(4 references)

产品编号 ABIN967494

发货至: 中国

Datasheet as PDF

Quick Overview for MAP2 抗体 (ABIN967494)

抗原

See all MAP2 抗体

MAP2 (Microtubule-Associated Protein 2 (MAP2))

适用

人, 大鼠, Cow, Quail, Frog

宿主

小鼠

克隆类型

单克隆

标记

This MAP2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

Ap20

Discover our top product MAP2 Primary Antibody

品牌

BD Pharmingen™

交叉反应

人, Frog, Quail, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Cow MAP2

亚型

IgG1
anti-Microtubule-Associated Protein 2 (MAP2) (AA 260-559) antibody
MAP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) antibody
MAP2 适用: 人, 小鼠, Pig WB, ELISA, ICC, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal MT-08 unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) antibody
MAP2 适用: Cow WB, ICC, IF 宿主: 小鸡 Polyclonal unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) (AA 350-400) antibody
MAP2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) antibody
Microarray verified MAP2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB573626 unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) (N-Term) antibody
MAP2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) (AA 727-985) antibody
MAP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) (AA 1459-1718) antibody
MAP2 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) antibody
Microarray verified MAP2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB2355 unconjugated

anti-Microtubule-Associated Protein 2 (MAP2) antibody
Microarray verified MAP2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB146071 unconjugated

应用备注

Applications include western blot analysis (1-2 µg/ml), immunoprecipitation, and immunohistochemistry of paraformaldehyde-fixed tissue cultured cells, and frozen tissue sections. T98G human glioblastoma cells (ATCC CRL-1690) and rat brain enriched microtubule protein preparations are suggested as positive controls.

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Kalcheva, Albala, Binder, Shafit-Zagardo: "Localization of specific epitopes on human microtubule-associated protein 2." in: Journal of neurochemistry, Vol. 63, Issue 6, pp. 2336-41, (1994) (PubMed).

Tucker: "The roles of microtubule-associated proteins in brain morphogenesis: a review." in: Brain research. Brain research reviews, Vol. 15, Issue 2, pp. 101-20, (1991) (PubMed).

Tucker, Binder, Viereck, Hemmings, Matus: "The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 12, pp. 4503-12, (1989) (PubMed).

Binder, Frankfurter, Rebhun: "Differential localization of MAP-2 and tau in mammalian neurons in situ." in: Annals of the New York Academy of Sciences, Vol. 466, pp. 145-66, (1986) (PubMed).
抗原

MAP2 (Microtubule-Associated Protein 2 (MAP2))

别名

MAP2

背景

Microtubule-associated protein 2 (MAP2) is a neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. It is expressed in the cell body and dendrites of neurons, but is absent in neuronal processes. The expression of MAP2 is developmentally regulated, and there are multiple high and low molecular weight isoforms, all derived from one MAP2 gene. MAP2a, MAP2b, and MAP2c are the three major MAP2 isoforms. The high molecular weight MAP2 isoforms, MAP2a and MAP2b consist of a large projection arm and a short microtubule binding domain. The low molecular weight MAP-2 isoform, MAP2c, lacks most of the projection arm of the high molecular weight isoforms. MAP2c is the earliest expressed MAP2 and is derived by alternative splicing MAP2a and MAP2b are considered to be adult expressed MAPs, with MAP2a expression occuring later in development than MAP2b expression. MAP2a is thought to result from a post-translational modification of MAP2b. High molecular weight MAP2 isoforms (2a and 2b) migrate as a doublet at a molecular weight of >300 kDa. The low molecular weight MAP2 isoform 2c migrates at 70 kDa.
The Ap-20 antibody recognizes high molecular weight MAP2 isoforms MAP2a and MAP2b. Specifically, it has been shown to recognize MAP2a and MAP2b from human, bovine, rat, frog, and quail cells and tissues. Ap-20 does not recognize the low molecular weight MAP2 isoforms (MAP2c) or other microtubule proteins. Ap-20 reacts with an epitope between amino acids 997-1332 of high molecular weight MAP2 isoforms.4 Bovine MAP2 was used as immunogen.
Synonyms: a and b Isoforms

分子量

280 kDa