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MAP2 抗体

MAP2 适用: Cow WB, IF, ICC 宿主: 小鸡 Polyclonal unconjugated
产品编号 ABIN361345
发货至: 中国
  • 抗原 See all MAP2 抗体
    MAP2 (Microtubule-Associated Protein 2 (MAP2))
    适用
    • 149
    • 99
    • 93
    • 28
    • 25
    • 5
    • 5
    • 3
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    • 1
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    • 1
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    Cow
    宿主
    • 125
    • 47
    • 7
    • 2
    小鸡
    克隆类型
    • 135
    • 45
    多克隆
    标记
    • 92
    • 16
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    • 7
    • 6
    • 4
    • 4
    • 4
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    • 3
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    • 3
    • 2
    • 1
    This MAP2 antibody is un-conjugated
    应用范围
    • 126
    • 64
    • 57
    • 39
    • 39
    • 30
    • 29
    • 28
    • 22
    • 20
    • 17
    • 9
    • 4
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    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    特异性
    Specific for the ~ 280k MAP2 protein.
    交叉反应
    牛, 人, 小鼠, 大鼠
    灵敏度
    The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.
    纯化方法
    Total IgY fraction
    免疫原
    recombinant bovine MAP2 protein expressed in and purified from E. Coli
    Top Product
    Discover our top product MAP2 Primary Antibody
  • 应用备注
    Recommended Dilution: WB: 1:20,000 IF: 1:2,500 Quality Control: Western blots performed on each lot.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    total IgY fraction in PBS + 10 mM Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • Woike, Wang, Tibbe, Hassani Nia, Failla, Kibæk, Overgård, Larsen, Fagerberg, Barsukov, Kreienkamp: "Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders." in: Scientific reports, Vol. 12, Issue 1, pp. 902, (2022) (PubMed).

    Hassani Nia, Woike, Bento, Niebling, Tibbe, Schulz, Hirnet, Skiba, Hönck, Veith, Günther, Scholz, Bierhals, Driemeyer, Bend, Failla, Lohr, Alai, Kreienkamp: "Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans." in: Molecular psychiatry, (2022) (PubMed).

    Hassani Nia, Woike, Martens, Klüssendorf, Hönck, Harder, Kreienkamp: "Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus." in: Molecular autism, Vol. 11, Issue 1, pp. 85, (2021) (PubMed).

    Hassani Nia, Woike, Kloth, Kortüm, Kreienkamp: "Truncating mutations in SHANK3 associated with global developmental delay interfere with nuclear β-catenin signaling." in: Journal of neurochemistry, (2020) (PubMed).

    Ho, Dallalzadeh, Karathanasis, Keles, Vangala, Grogan, Poirazi, Martin: "GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons." in: Molecular and cellular neurosciences, Vol. 61, pp. 1-12, (2014) (PubMed).

  • 抗原
    MAP2 (Microtubule-Associated Protein 2 (MAP2))
    别名
    MAP2 (MAP2 产品)
    别名
    MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody
    背景
    Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ( (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ( (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). Anti-MAP2 Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Right:Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
    分子量
    '280 kDa
    基因ID
    4133
    UniProt
    P11137
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