(10 references)-
- 抗原 See all CD24 抗体
- CD24 (CD24 Molecule (CD24))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD24 antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- C3H/HeJ mouse thymocytes
- 克隆位点
- J11d
- 亚型
- IgM kappa
- Top Product
- Discover our top product CD24 Primary Antibody
-
anti-CD24 Molecule (CD24) antibody (FITC)
CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 FITC
anti-CD24 Molecule (CD24) (AA 15-80) antibodyCD24 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4F4E10 unconjugated
anti-CD24 Molecule (CD24) (AA 60-80) antibodyCD24 适用: 人, 小鼠, 大鼠 IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) (AA 1-80) antibodyCD24 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 FACS, IHC (fro), CyTOF 宿主: 小鼠 Monoclonal SN3 unconjugated
anti-CD24 Molecule (CD24) antibody (PE)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 PE
anti-CD24 Molecule (CD24) antibody (APC)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 APC
anti-CD24 Molecule (CD24) (AA 30-60) antibodyCD24 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 FACS, IHC, IHC (fro), IF 宿主: 小鼠 Monoclonal ML5 unconjugated
-
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4° C.
-
-
: "Isolation of murine natural killer cells." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.22, (2008) (PubMed).
: "Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin." in: International immunology, Vol. 7, Issue 10, pp. 1557-65, (1996) (PubMed).
: "Isolation, phenotype, and allostimulatory activity of mouse liver dendritic cells." in: Transplantation, Vol. 58, Issue 4, pp. 484-91, (1994) (PubMed).
: "Peripheral B cell maturation. II. Heat-stable antigen(hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4431-44, (1993) (PubMed).
: "The heat stable antigen (mouse CD24) gene is differentially regulated but has a housekeeping promoter." in: The Journal of biological chemistry, Vol. 268, Issue 31, pp. 23345-52, (1993) (PubMed).
: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).
: "Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies." in: European journal of immunology, Vol. 20, Issue 7, pp. 1597-602, (1990) (PubMed).
: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).
: "Expression and functional significance of the J11d marker on mouse thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 7, pp. 2013-8, (1987) (PubMed).
: "A monoclonal antibody discriminating between subsets of T and B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2496-501, (1982) (PubMed).
-
: "Isolation of murine natural killer cells." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.22, (2008) (PubMed).
-
- 抗原
- CD24 (CD24 Molecule (CD24))
- 别名
- CD24 (CD24 产品)
- 背景
-
The J11d antibody reacts with CD24 (Heat Stable Antigen, HSA or HsAg), a variably glycosylated membrane protein found on erythrocytes, granulocytes, monocytes, and lymphocytes. Levels of expression of CD24 vary during differentiation of the T and B cell lineages: Immature B cells in the bone marrow and spleen of adult mice express high levels of CD24, whereas mature peripheral B cells express intermediate levels of CD24. Similarly, the majority of thymocytes express high levels of CD24, while mature thymic and peripheral T cells do not express CD24. Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24. CD24 is not expressed by NK cells. CD24 is a ligand of CD62P (P-selectin). In vitro treatment of splenocytes with J11d antibody plus complement reduces primary IgM responses and proliferative responses to LPS. While the monoclonal antibodies 30-F1, M1/69 (ABIN967572), and J11d all react with CD24, they show subtle differences in the level of staining of different cell populations. When possible, investigators should continue to use the same monoclonal antibody as used in previous studies.
Synonyms: Heat Stable Antigen - 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
-
You are here:
