Clusterin 抗体
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- 抗原 See all Clusterin (CLU) 抗体
- Clusterin (CLU)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Clusterin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- E5
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CLU Primary Antibody
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anti-Clusterin (CLU) (AA 331-449) antibody
CLU 适用: 人, 大鼠, 小鼠 ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 229-446) antibodyCLU 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-448) antibodyCLU 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 228-449) antibodyCLU 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 226-447) antibodyCLU 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D4 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 人 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 23-120) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-447) antibodyCLU 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Clusterin (CLU) (AA 402-501) antibodyCLU 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A11 unconjugated
anti-Clusterin (CLU) (Internal Region) antibodyCLU 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry:
The E5 antibody, specific for human clusterin, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Tissue tested was human tonsil. The antibody stains dendritic cells in the follicle and basement membrane of epithelial cells. The isotype control recommended for use with this antibody is purified mouse IgG1. For optimal indirect immunohistochemical staining, the E5 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Mouse Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate, can be used for staining. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas." in: Blood, Vol. 96, Issue 2, pp. 398-404, (2000) (PubMed).
: "Clusterin: physiologic and pathophysiologic considerations." in: The international journal of biochemistry & cell biology, Vol. 27, Issue 7, pp. 633-45, (1995) (PubMed).
: "SP-40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis." in: The Journal of clinical investigation, Vol. 81, Issue 6, pp. 1858-64, (1988) (PubMed).
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: "Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas." in: Blood, Vol. 96, Issue 2, pp. 398-404, (2000) (PubMed).
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- 抗原
- Clusterin (CLU)
- 别名
- Clusterin (CLU 产品)
- 背景
- The E5 antibody reacts with human clusterin. Clusterin was originally isolated and characterized from glomerular immune deposits of patients with renal glomerulonephritis. Clusterin, an 80 kD disulfide-linked, heterodimeric glycoprotein (previously named SP-40,40 [serum protein 40 kD,40kD]) was shown to be part of the SC5b-9 complex of complement, yet separate and distinct from the S protein. Clusterins have since been identified in a number of mammalian species and have been implicated in diverse cellular activities, however, the exact role remains to be elucidated.
- 途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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