FLT4 抗体 (Extracellular Domain)
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北京 101111
Quick Overview for FLT4 抗体 (Extracellular Domain) (ABIN967368)
抗原
See all FLT4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- VEGFR-3 extracellular domain
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亚型
- IgG2a kappa
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anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibody
FLT4 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ICC, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 25-259) antibodyFLT4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FACS, ICC, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1-439) antibodyFLT4 适用: 人 WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 818CT12-1-1 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibodyFLT4 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 818CT12-1-1 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 901-1000) antibodyFLT4 适用: 人, 小鼠, Cow ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibodyFLT4 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 34-133) antibodyFLT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5B5 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibodyFLT4 适用: 人 IHC (p) 宿主: 小鼠 Monoclonal 8C1 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibodyFLT4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1112-1329) antibodyFLT4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry:
The AFL4 antibody, specific for mouse VEGFR-3, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Antigen retrieval with Retrievagen A is recommended for formalin-fixed, paraffin sections. Tissues tested were mouse spleen, liver, kidney, lung and small intestine. The antibody stains endothelial cells of lymphatic vessels. The isotype control recommended for use with this antibody is purified rat IgG2akappa. For optimal indirect immunohistochemical staining, the AFL4 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Rat Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate, can be used for staining. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "VEGF-D promotes the metastatic spread of tumor cells via the lymphatics." in: Nature medicine, Vol. 7, Issue 2, pp. 186-91, (2001) (PubMed).
: "Involvement of vascular endothelial growth factor receptor-3 in maintenance of integrity of endothelial cell lining during tumor angiogenesis." in: Blood, Vol. 96, Issue 2, pp. 546-53, (2000) (PubMed).
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: "VEGF-D promotes the metastatic spread of tumor cells via the lymphatics." in: Nature medicine, Vol. 7, Issue 2, pp. 186-91, (2001) (PubMed).
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- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
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别名
- VEGFR-3
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背景
- The AFL4 antibody reacts with mouse VEGFR-3, an endothelial-specific receptor tyrosine kinase (RTK) related to VEGF receptors. The immunogen used was the extracellular domain of VEGFR-3, corresponding to nucleotides 45-2354 in the GeneBank L07296. VEGFR-3 is induced in all endothelial cells (EC’s) during early embryogenesis, and its expression eventually disappears from the vascular endothelial cells of adult tissues. VEGFR-3 is constitutively expressed in the adult lymphatic endothelium. Although VEGFR-3 is not expressed in adult blood vessels, it is induced in vascular endothelial cells of tumor-bearing tissues. Studies have shown that both VEGF-C and VEGF-D, ligands for VEGFR-3, promote lymphatic vessel proliferation throughout central tumor areas and at their peripheries, demonstrating that such molecules aid in the spread of tumor cells from the primary tumor to local lymph nodes.
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途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling
抗原
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