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GZMB 抗体

Name: GZMB 抗体
SKU: ABIN967291
Availability: OutOfStock

This anti-GZMB antibody is a 小鼠单克隆 antibody detecting GZMB in WB. Suitable for 人和大鼠. This Primary Antibody has been cited in 7+ publications.

Western blot analysis of Granzyme B. (GZMB 抗体)

1 image

(7 references)

产品编号 ABIN967291

发货至: 中国

Datasheet as PDF

Quick Overview for GZMB 抗体 (ABIN967291)

抗原

See all GZMB 抗体

GZMB (Granzyme B (GZMB))

适用

人, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This GZMB antibody is un-conjugated

应用范围

Western Blotting (WB)

克隆位点

2C5-F5

Discover our top product GZMB Primary Antibody

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

亚型

IgG2a
anti-Granzyme B (GZMB) (AA 21-247) antibody
GZMB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Granzyme B (GZMB) (AA 73-187) antibody
GZMB 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal GZMB-3014 unconjugated

anti-Granzyme B (GZMB) (AA 21-130) antibody
GZMB 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Granzyme B (GZMB) (AA 1-247) antibody
GZMB 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Granzyme B (GZMB) (AA 73-187) antibody
GZMB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GZMB-3056 unconjugated

anti-Granzyme B (GZMB) antibody
GZMB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Granzyme B (GZMB) antibody
GZMB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CLB-GB11 unconjugated

anti-Granzyme B (GZMB) (AA 21-247) antibody
GZMB 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 4E4 unconjugated

anti-Granzyme B (GZMB) (AA 21-247) antibody
GZMB 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2G12G7 unconjugated

anti-Granzyme B (GZMB) antibody
GZMB 适用: 人 ELISA, FACS, cELISA 宿主: 小鼠 Monoclonal GM-4C1 unconjugated

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Pinkoski, Waterhouse, Heibein, Wolf, Kuwana, Goldstein, Newmeyer, Bleackley, Green: "Granzyme B-mediated apoptosis proceeds predominantly through a Bcl-2-inhibitable mitochondrial pathway." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 12060-7, (2001) (PubMed).

Rotonda, Garcia-Calvo, Bull, Geissler, McKeever, Willoughby, Thornberry, Becker: "The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1." in: Chemistry & biology, Vol. 8, Issue 4, pp. 357-68, (2001) (PubMed).

Sharif-Askari, Alam, Rhéaume, Beresford, Scotto, Sharma, Lee, DeWolf, Nuttall, Lieberman, Sékaly: "Direct cleavage of the human DNA fragmentation factor-45 by granzyme B induces caspase-activated DNase release and DNA fragmentation." in: The EMBO journal, Vol. 20, Issue 12, pp. 3101-13, (2001) (PubMed).

Barry, Heibein, Pinkoski, Lee, Moyer, Green, Bleackley: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, (2000) (PubMed).

Beresford, Xia, Greenberg, Lieberman: "Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation." in: Immunity, Vol. 10, Issue 5, pp. 585-94, (1999) (PubMed).

Shresta, Graubert, Thomas, Raptis, Ley: "Granzyme A initiates an alternative pathway for granule-mediated apoptosis." in: Immunity, Vol. 10, Issue 5, pp. 595-605, (1999) (PubMed).

Andrade, Roy, Nicholson, Thornberry, Rosen, Casciola-Rosen: "Granzyme B directly and efficiently cleaves several downstream caspase substrates: implications for CTL-induced apoptosis." in: Immunity, Vol. 8, Issue 4, pp. 451-60, (1998) (PubMed).
抗原

GZMB (Granzyme B (GZMB))

别名

Granzyme B

背景

The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a poreforming protein, perforin, and a group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic pathway, granzyme B is activated immediately, while granzyme A acts hours later. The physiological substrates for granzyme A in the apoptotic pathway have not been identified. Studies involving mice which are deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with the granzyme A pathway acting as a backup. Granzyme B has been shown to induce apoptosis and to cleave a number of substrates which are similar in specificity to those of the caspase family of proteinases. Granzyme B can cleave substrates, such as DNA-PKcs, and nuclear mitotic apparatus protein (NuMA). Furthermore, Granzyme B can also cleave substrates such as Bid and DFF45 in a caspase-independent fashion. However, further research is needed to delineate the exact role of caspases in cytotoxic T lymphocyte-induced apoptosis involving Granzyme B. Granzyme B migrates at approximately 32 kDa in SDS/PAGE. Clone 2C5/F5 recognizes human and rat granzyme B.

分子量

32 kDa

途径

Apoptosis, Caspase Cascade in Apoptosis