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RAB25 抗体

This anti-RAB25 antibody is a 单克隆 antibody detecting RAB25 in ELISA, IHC 和 WB. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN966930
发货至: 中国

Quick Overview for RAB25 抗体 (ABIN966930)

抗原

See all RAB25 抗体
RAB25 (RAB25, Member RAS Oncogene Family (RAB25))

适用

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宿主

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克隆类型

  • 49
  • 9
单克隆

标记

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This RAB25 antibody is un-conjugated

应用范围

  • 44
  • 25
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ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
  • 特异性

    Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3)

    纯化方法

    Antibodies are purified by protein A affinity chromatography

    亚型

    IgG2a
  • 应用备注

    Western Blot: 1: 500- 1: 2,000
    IHC(P): 1: 500- 1: 2,000
    IHC(F): 1: 500- 1: 2,000
    ELISA: Propose dilution 1: 10,000.
    Determining optimal working dilutions by titration test.

    限制

    仅限研究用
  • 缓冲液

    PBS with 0.2%BSA

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C
  • Goldenring, Shen, Vaughan, Modlin: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, (1993) (PubMed).

  • 抗原

    RAB25 (RAB25, Member RAS Oncogene Family (RAB25))

    别名

    Rab25

    背景

    Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

    基因ID

    57111
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