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NUMA1 抗体 (Cytoplasmic, Nuclear)

NUMA1 适用: 人 ELISA, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 3-3A unconjugated
产品编号 ABIN966706
发货至: 中国
  • 抗原 See all NUMA1 抗体
    NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
    抗原表位
    • 15
    • 15
    • 7
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic, Nuclear
    适用
    • 80
    • 19
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 68
    • 12
    小鼠
    克隆类型
    • 69
    • 11
    单克隆
    标记
    • 34
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This NUMA1 antibody is un-conjugated
    应用范围
    • 28
    • 28
    • 26
    • 17
    • 16
    • 15
    • 13
    • 8
    • 8
    • 6
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.
    纯化方法
    Concentrated.
    免疫原
    Live Ls 174T cells (colon carcinoma).
    克隆位点
    3-3A
    亚型
    IgM
    Top Product
    Discover our top product NUMA1 Primary Antibody
  • 应用备注
    Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
    Western Blot 1:100-1:300.
    Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
    说明

    Recommended Positive Control: Tonsil, Thymus, Spleen

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/ml.
    缓冲液
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • Gueth-Hallonet, Weber, Osborn: "Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).

  • 抗原
    NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
    别名
    NuMA (NUMA1 产品)
    分子量
    228 kDa
    基因ID
    4926
    途径
    Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase
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