NUMA1 抗体 (Cytoplasmic, Nuclear)
(1 reference)Quick Overview for NUMA1 抗体 (Cytoplasmic, Nuclear) (ABIN966706)
抗原
See all NUMA1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Cytoplasmic, Nuclear
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特异性
- Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.
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纯化方法
- Concentrated.
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免疫原
- Live Ls 174T cells (colon carcinoma).
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亚型
- IgM
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anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 1-309) antibody
NUMA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (N-Term) antibodyNUMA1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 627-870) antibodyNUMA1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 1-1193) antibodyNUMA1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 1-1193) antibodyNUMA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 200-306) antibodyNUMA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1C5 unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) antibodyNUMA1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (N-Term) antibodyNUMA1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 1700-2115) antibodyNUMA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) (AA 1700-2115) antibodyNUMA1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D9 unconjugated
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应用备注
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Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
Western Blot 1:100-1:300.
Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory. -
说明
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Recommended Positive Control: Tonsil, Thymus, Spleen
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/ml.
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缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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: "Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).
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: "Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).
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- NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
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别名
- NuMA
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分子量
- 228 kDa
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基因ID
- 4926
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途径
- Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase
抗原
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