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NEUROFILAMENTS 抗体

适用: 人, 小鼠, 大鼠 IHC (fp), IP, WB 宿主: 小鼠 Monoclonal 2F11 unconjugated
产品编号 ABIN966644
发货至: 中国
  • 抗原
    NEUROFILAMENTS
    适用
    人, 小鼠, 大鼠
    宿主
    小鼠
    克隆类型
    单克隆
    应用范围
    Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), Western Blotting (WB)
    纯化方法
    Concentrated.
    免疫原
    Neurofilament from Human Brain
    克隆位点
    2F11
    亚型
    IgG1 kappa
  • 应用备注
    Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100
    WB: 1:100-1:200.
    Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 in IHC . The optimal conditions should be determined by the individual laboratory.
    说明

    Cellular Localization: Cytoplasmic.
    Recommended Positive Control: Human brain, IMR-5 cells

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.25 mg/ml.
    缓冲液
    20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • Baldinger: "Sodium hyaluronate used to correct lens nucleus tumble." in: Ophthalmic surgery, Vol. 16, Issue 9, pp. 590, (1985) (PubMed).

  • 抗原
    NEUROFILAMENTS
    背景
    This antibody reacts with the 70 and 200 kD polypeptides of neurofilament. This antibody recognizes neurons, neuronal processes, and peripheral nerves, as well as, sympathetic ganglion cells and adrenal medulla. Cell body of neurons containing the non-phosphorylated neurofilamentis weakly stained.
    分子量
    68 kDa, 200 kDa(phosphorylated form)
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